Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Monoclonal Antibody (CIP1/823 + DCS-6.2).
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p21 Monoclonal Antibody (CIP1/823 + DCS-6.2).

Anti-p21WAF1 Antibody Clone CIP1/823 + DCS-60.2

$ 329.00
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SKU: 1026-MSM5
Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1026-MSM5-P1
p21WAF1 General Information
Alternate Names
cyclin-dependent kinase inhibitor 1, CDK-interacting protein 1, p21Cip1, p21 Cip1, p21Waf1, p21 Waf1
Molecular Weight
21kDa
Chromosomal Location
6p21.31
Curated Database and Bioinformatic Data
Gene SymbolCDKN1A
Entrez Gene ID1026
Ensemble Gene IDENSG00000124762
RefSeq Protein Accession(s)NP_001207706, NP_000380, NP_001207707, NP_001278478, NP_510867
RefSeq mRNA Accession(s)NM_001291549, NM_001220778, NM_000389, NM_078467, NM_001220777
RefSeq Genomic Accession(s)NG_009364, NC_000006, NC_018917
UniProt ID(s)A0A024RCX5, P38936
UniGene ID(s)A0A024RCX5, P38936
HGNC ID(s)1784
Cosmic ID(s)CDKN1A
KEGG Gene ID(s)hsa:1026
PharmGKB ID(s)PA104
General Description of p21WAF1.
This MAb recognizes a 21kDa protein, identified as the p21WAF1 tumor suppressor protein. This MAb is highly specific to p21, shows no cross-reaction with other closely related mitotic inhibitors. p21WAF1 is a specific inhibitor of cdks, a tumor suppressor involved in the pathogenesis of a variety of malignancies. The expression of this gene acts as an inhibitor of the cell cycle during G1 phase, is tightly controlled by the tumor suppressor protein p53. Its expression is induced by the wild type, but not mutant, p53 suppressor protein. Normal cells generally display a rather intense nuclear p21 expression. Loss of p21 expression has been reported in many carcinomas (gastric carcinoma, non-small cell lung carcinoma, thyroid carcinoma).

Human, Mouse (-), and Rat (-) Anti-p21WAF1 Antibody Product Attributes

Species: Human, Mouse (-), and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-p21WAF1 Antibody Clone: CIP1/823 + DCS-60.2
Clone CIP1/823 + DCS-60.2 Host and Isotype: Mouse IgG
Anti-Human, Mouse (-), and Rat (-) p21WAF1 Positive Control Sample: HeLa Cells. Skin, colon, or breast carcinoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human CDKN1A protein (CIP1/823); Human recombinant p21 protein (DCS-60.2)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

p21WAF1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CDKN1A antibody staining is expected to be primarily localized to the nuclear bodies and nucleoplasm. There is variability in either the signal strength or the localization of signal in nuclear bodies, and nucleoplasm from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in p21WAF1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in Leydig cells in the testis, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine and vagina and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in Leydig cells in the testis, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine and vagina and trophoblastic cells in the placenta. Low, but measureable presence of p21WAF1 could be seen in cells in the glomeruli in kidney, cells in the red pulp in spleen, cells in the tubules in kidney, exocrine glandular cells in the pancreas, germinal center cells in the lymph node, glandular cells in the breast, fallopian tube, gallbladder, parathyroid gland, prostate, seminal vesicle and stomach, hematopoietic cells in the bone marrow, hepatocytes in liver, keratinocytes in skin, non-germinal center cells in the lymph node and tonsil and respiratory epithelial cells in the nasopharynx. We were unable to detect p21WAF1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of p21WAF1 expression as measured by anti-p21WAF1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - +++ + ++ - ++ - + - + - + - + ++ + - + ++
CDKN1A Variability ++ ++ ++ +++ ++ ++ ++ + ++ + +++ ++ ++ + +++ ++ ++ + ++ ++

Limitations and Warranty

enQuire Bio's p21WAF1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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