Flow Cytometry of human p27 on HeLa Cells. Black: Cells alone; Grey: Isotype Control; Green: Alexa Fluor® 488-labeled p27 Monoclonal Antibody (SX53G8).
Flow Cytometry of human p27 on HeLa Cells. Black: Cells alone; Grey: Isotype Control; Green: Alexa Fluor® 488-labeled p27 Monoclonal Antibody (SX53G8).

Anti-p27Kip1 Antibody Clone SX53G8

$ 329.00
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SKU: 1027-MSM1
Species: Human, Mouse, Rat, and Monkey
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1027-MSM1-P1
p27Kip1 General Information
Alternate Names
Cyclin-dependent kinase inhibitor 1B, p27Kip1, CDKN1B, p27Kip1, p27 Kip1
Molecular Weight
25-26kDa
Chromosomal Location
12p13.1
Curated Database and Bioinformatic Data
Gene SymbolCDKN1B
Entrez Gene ID1027
Ensemble Gene IDENSG00000111276
RefSeq Protein Accession(s)NP_004055
RefSeq mRNA Accession(s)NM_004064
RefSeq Genomic Accession(s)NG_016341, NC_018923, NC_000012
UniProt ID(s)P46527, Q6I9V6
UniGene ID(s)P46527, Q6I9V6
HGNC ID(s)1785
Cosmic ID(s)CDKN1B
KEGG Gene ID(s)hsa:1027
PharmGKB ID(s)PA105
General Description of p27Kip1.
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific, shows no cross-reaction with other related mitotic inhibitors. In Western Blot of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression, has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma,, lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.

Human, Mouse, Rat, and Monkey Anti-p27Kip1 Antibody Product Attributes

Species: Human, Mouse, Rat, and Monkey
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-p27Kip1 Antibody Clone: SX53G8
Clone SX53G8 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, Rat, and Monkey p27Kip1 Positive Control Sample: ZR75, T47D, SK-BR-3, MDA-MB-231, HeLa or MCF7 cells. Tonsil, Breast, Cervical or Colon Carcinoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified GST-p27 fusion protein of human origin
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

p27Kip1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CDKN1B antibody staining is expected to be primarily localized to the nucleus and vesicles. There is variability in either the signal strength or the localization of signal in nucleus from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in p27Kip1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, cells in the granular layer in cerebellum, follicle cells in the ovary, glandular cells in the cervix, uterine and fallopian tube, hematopoietic cells in the bone marrow, neuronal cells in the caudate nucleus and hippocampus, non-germinal center cells in the tonsil, ovarian stroma cells in the ovary, pneumocytes in lung and Purkinje cells in the cerebellum. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, cells in the granular layer in cerebellum, follicle cells in the ovary, glandular cells in the cervix, uterine and fallopian tube, hematopoietic cells in the bone marrow, neuronal cells in the caudate nucleus and hippocampus, non-germinal center cells in the tonsil, ovarian stroma cells in the ovary, pneumocytes in lung and Purkinje cells in the cerebellum. Low, but measureable presence of p27Kip1 could be seen inadipocytes in breast and mesenchymal tissue, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, cells in the tubules in kidney, cells in the white pulp in spleen, decidual cells in the placenta, endothelial cells in the cerebral cortex and colon, exocrine glandular cells in the pancreas, fibroblasts in mesenchymal tissue, germinal center cells in the lymph node and tonsil, glandular cells in the appendix, breast, colon, duodenum, epididymis, parathyroid gland, rectum, seminal vesicle, small intestine, stomach and thyroid gland, hepatocytes in liver, Langerhans in skin, Leydig cells in the testis, macrophages in lung, myocytes in heart muscle, myoepithelial cells in the breast, neuropil in cerebral cortex, respiratory epithelial cells in the bronchus, smooth muscle cells in the smooth muscle and squamous epithelial cells in the esophagus. We were unable to detect p27Kip1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of p27Kip1 expression as measured by anti-p27Kip1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + + - + - ++ ++ - + - + - - + - - + - ++ -
CDKN1B Variability ++ +++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ + + ++ + ++ +

Limitations and Warranty

enQuire Bio's p27Kip1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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