Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
|Recommended Dilution (Conc)||1:100-1:200|
|Pretreatment||Citrate Buffer pH 6.0|
|Incubation Parameters||30 min at Room Temperature|
Anti-p27Kip1 Antibody Clone: EP104
Host and Isotype: Rabbit Rabbit IgG
Recommended Positive Control Sample: Tonsil for normal tissue and colorectal cancer for tumor tissue
Cellular Localization of Antibody EP104 Staining: Nuclear/Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: A synthetic peptide corresponding to amino acid residues in C-terminal end of human p27Kip1 is used as an immunogen.
Storage Conditions: This antibody should be stored refrigerated (2-8Â°C). This product should not be used past the expiration date printed on the vial.
p27Kip1 Information for Pathologists
Summary:Cyclin dependent kinase inhibitor leading to cell cycle arrest in G1 phase. Member of Cip/Kip family of proteins. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung. Interpretation Nuclear stain.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.