Immunohistochemical staining of p27Kip1 of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

p27Kip1 [EP104] Antibody (cGMP)-DISC

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SKU: QIVD169
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Rabbit IgG

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: QIVD169-0.1ml

p27Kip1 General Information
Alternate Names

Molecular Weight
22.1 kDa
Chromosomal Location
p13.1 [chr: 12] [chr_start: 12715058] [chr_end: 12722369] [strand: 1]
Curated Database and Bioinformatic Data
Gene Symbol	CDKN1B
Entrez Gene ID	1027
RefSeq Protein Accession(s)	NP_004055
RefSeq mRNA Accession(s)	; NM_004064
RefSeq Genomic Accession(s)	NG_016341; NC_000012
UniProt ID(s)	P46527
PharmGKB ID(s)	PA105
KEGG Gene ID(s)	hsa:1027
Associated Diseases (KEGG IDs)	Multiple endocrine neoplasia 4 (MEN4) [MIM:610755]: Multiple endocrine neoplasia (MEN) syndromes are inherited cancer syndromes of the thyroid. MEN4 is a MEN-like syndrome with a phenotypic overlap of both MEN1 and MEN2. {ECO:0000269\|PubMed:17030811}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of p27Kip1 .
p27Kip1 is a cyclin-dependent kinase inhibitor involved in G1 arrest. p27Kip1 binds to and inhibits CyclinE-Cdk2 complex, cyclinA-CDK2 and cyclinD1-CDK4 complexes. P27Kip1 is regulated by phosphorylation and the site of phosphorylation determines the fate of the protein, whether it is degraded or exported out of the nucleus to the cytoplasm. The expression of p27Kip1 is high in normal cells. Down regulation of p27Kip1 is found in many types of cancer, and decreased expression of p27Kip1 is associated with poor prognosis in several tumors, including lung, breast, colorectal and prostate.

Antibody DISCONTINUED

Sample Type: FFPE Patient Samples.

Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.

Application Notes

Specification	Recommendation
Recommended Dilution (Conc)	1:100-1:200
Pretreatment	Citrate Buffer pH 6.0
Incubation Parameters	30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal

Anti-p27Kip1 Antibody Clone: EP104

Host and Isotype: Rabbit Rabbit IgG

Recommended Positive Control Sample: Tonsil for normal tissue and colorectal cancer for tumor tissue

Cellular Localization of Antibody EP104 Staining: Nuclear/Cytoplasmic

Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN₃

Antibody Concentration: Lot specific. Plese contact tech support for data.

Immunogen: A synthetic peptide corresponding to amino acid residues in C-terminal end of human p27Kip1 is used as an immunogen.

Storage Conditions: This antibody should be stored refrigerated (2-8Â°C). This product should not be used past the expiration date printed on the vial.

p27Kip1 Information for Pathologists

Summary:

Cyclin dependent kinase inhibitor leading to cell cycle arrest in G1 phase. Member of Cip/Kip family of proteins. Loss or reduced expression is associated with poor outcome in carcinoma of breast, prostate, GI tract and lung. Interpretation Nuclear stain.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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