Data from SDS-PAGE analysis of Anti-p53 Tumor Suppressor Protein antibody (Clone TP53/1799R). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Western blot analysis of Hela cell lysate using p53 Recombinant Rabbit Monoclonal Antibody (TP53/1799R).

Anti-p53 Tumor Suppressor Protein Antibody Clone TP53/1799R

$ 199.00$ 549.00
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SKU: 7157-RBM10
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Rabbit IgG kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7157-RBM10-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
7157-RBM10-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
7157-RBM10-P2 Size: 200 ug, Tag: Unconjugated $ 549.00
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SKU: 7157-RBM10-P0

Human Anti-p53 Tumor Suppressor Protein Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Recombinant Monoclonal
Anti-p53 Tumor Suppressor Protein Antibody Clone: TP53/1799R
Clone TP53/1799R Host and Isotype: Rabbit IgG kappa
Anti-Human p53 Tumor Suppressor Protein Positive Control Sample: MDA-MB-231 Cells. Breast or Colon carcinoma
Cellular Localization of Antibody Predominantly nuclear
Buffer and Stabilizer: 10mM PBS with or without 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human TP53 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

p53 Tumor Suppressor Protein Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TP53 antibody staining is expected to be primarily localized to the nucleoplasm. There is variability in either the signal strength or the localization of signal in nucleoplasm from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in p53 Tumor Suppressor Protein antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of p53 Tumor Suppressor Protein could be seen inglandular cells in the adrenal gland, hematopoietic cells in the bone marrow, glandular cells in the breast, respiratory epithelial cells in the bronchus, neuronal cells in the caudate nucleus, glandular cells in the cervix, uterine and endometrium, cells in the glomeruli in kidney, islets of Langerhans in pancreas, glandular cells in the parathyroid gland, decidual cells in the placenta, glandular cells in the salivary gland, fibroblasts in skin, cells in the white pulp in spleen, non-germinal center cells in the tonsil and squamous epithelial cells in the vagina. We were unable to detect p53 Tumor Suppressor Protein in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of p53 Tumor Suppressor Protein expression as measured by anti-p53 Tumor Suppressor Protein antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - + + - - - - + - + - ++ - - + + + - ++
TP53 Variability + + ++ ++ + ++ ++ ++ ++ ++ +++ ++ ++ + + ++ ++ ++ + +++

Limitations and Warranty

enQuire Bio's p53 Tumor Suppressor Protein Recombinant Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-p53 Tumor Suppressor Protein Antibody Clone TP53/1799R Tumor Suppressor Protein Specificity Assay (Array)-300x120.jpg IHC human Colon Carcinoma (TP53 1799R)-300x225.jpg Tumor Suppressor Protein(TP53 1799R) Gel-212x300.jpg Western Hela (TP53 1799)-103x300.jpg