Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p57 Monoclonal Antibody (57P6).
Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with p57 Monoclonal Antibody (57P6).

Anti-p57Kip2 Antibody Clone 57P06

$ 329.00
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SKU: 1028-MSM4
Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 1028-MSM4-P1
p57Kip2 General Information
Alternate Names
Cyclin-dependent kinase inhibitor 1C, p57, Kip2, p57 Kip2, CDKN1C
Molecular Weight
57kDa
Chromosomal Location
11p15.5
Curated Database and Bioinformatic Data
Gene SymbolCDKN1C
Entrez Gene ID1028
Ensemble Gene IDENSG00000129757, ENSG00000273707
RefSeq Protein Accession(s)XP_016872577, NP_000067, NP_001116102, NP_001116103, XP_005252789
RefSeq mRNA Accession(s)XM_005252732, XM_017017088 NM_000076, NM_001122630, NM_001122631
RefSeq Genomic Accession(s)NG_187585, NG_008022, NC_018922, NC_000011
UniProt ID(s)P49918
UniGene ID(s)P49918
HGNC ID(s)1786
Cosmic ID(s)CDKN1C
KEGG Gene ID(s)hsa:1028
PharmGKB ID(s)PA26320
General Description of p57Kip2.
Recognizes a protein of 57kDa, identified as p57Kip2. It shows no cross-reaction with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes,, is a negative regulator of cell proliferation. Anti-p57 has been used as an aide in identification of complete hydatidiform mole (CHM) (no nuclear labeling of cytotrophoblasts, stromal cells) from partial hydatidiform mole (PHM) in which both cytotrophoblasts, stromal cells stain. The histological differentiation of complete mole, partial mole,, hydropic spontaneous abortion is problematic. Most complete hydatidiform moles are diploid, whereas most partial moles are triploid. Ploidy studies will identify partial moles, but will not differentiate complete moles from non-molar gestations. Complete moles carry a high risk of persistent disease, choriocarcinoma, while partial moles have a very low risk. In normal placenta, many cytotrophoblast nuclei, stromal cells are labeled with this antibody. Similar findings apply to PHM, hydropic abortus tissues. Intervillous trophoblastic islands (IVTIs) demonstrate nuclear labeling in all three entities, serve as an internal control.

Human and Mouse Anti-p57Kip2 Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-p57Kip2 Antibody Clone: 57P06
Clone 57P06 Host and Isotype: Mouse IgG2b kappa
Anti-Human and Mouse p57Kip2 Positive Control Sample: LS174T, Raji, HT29, SK-BR3 cells. Colon or Prostate carcinomas.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human p57Kip2 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

p57Kip2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CDKN1C antibody staining is expected to be primarily localized to the cytosol, nuclear bodies and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in p57Kip2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the glomeruli in kidney, decidual cells in the placenta and trophoblastic cells in the placenta. More moderate antibody staining intensity was present in cells in the glomeruli in kidney, decidual cells in the placenta and trophoblastic cells in the placenta. Low, but measureable presence of p57Kip2 could be seen inglandular cells in the duodenum and small intestine and islets of Langerhans in pancreas. We were unable to detect p57Kip2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of p57Kip2 expression as measured by anti-p57Kip2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - + - - + - - + - - + - - - + - + + +
CDKN1C Variability ++ ++ ++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's p57Kip2 Anti-Human, Mouse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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