Immunohistochemical staining of p57Kip2  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

p57Kip2 [57P06] Antibody (cGMP).

$ 135.00$ 755.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD171
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2b, kappa

Product NumberDescriptionPrice
QIVD171-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD171-0.5ml Size: 0.5 ml, Format: Concentrate $ 445.00
QIVD171-1ml Size: 1 ml, Format: Concentrate $ 755.00
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SKU: QIVD171-0.1ml
p57Kip2 General Information
Alternate Names
Molecular Weight
32.2 kDa
Chromosomal Location
p15.4 [chr: 11] [chr_start: 2883213] [chr_end: 2885773] [strand: -1]; [chr: CHR_HSCHR11_1_CTG7] [chr_start: 2883205] [chr_end: 2885904] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolCDKN1C
Entrez Gene ID1028
RefSeq Protein Accession(s)NP_001116102; NP_000067; NP_001116103; XP_016872577
RefSeq mRNA Accession(s)NM_001362474; NM_001362475; NM_000076; NM_001122630; NM_001122631
RefSeq Genomic Accession(s)NT_187585; NG_008022; NC_000011
UniProt ID(s)P49918
PharmGKB ID(s)PA26320
KEGG Gene ID(s)hsa:1028
Associated Diseases (KEGG IDs)Beckwith-Wiedemann syndrome (BWS) [MIM:130650]: A disorder characterized by anterior abdominal wall defects including exomphalos (omphalocele), pre- and postnatal overgrowth, and macroglossia. Additional less frequent complications include specific developmental defects and a predisposition to embryonal tumors. {ECO:0000269|PubMed:10424811, ECO:0000269|PubMed:26077438}. The disease is caused by mutations affecting the gene represented in this entry.; Intrauterine growth retardation, metaphyseal dysplasia, adrenal hypoplasia congenita, and genital anomalies (IMAGE) [MIM:614732]: A rare condition characterized by intrauterine growth restriction, metaphyseal dysplasia, congenital adrenal hypoplasia, and genital anomalies. Patients with this condition may present shortly after birth with severe adrenal insufficiency, which can be life-threatening if not recognized early and commenced on steroid replacement therapy. Other reported features in this condition include, hypercalciuria and/or hypercalcemia, craniosynostosis, cleft palate, and scoliosis. {ECO:0000269|PubMed:22634751}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of p57Kip2 .
This antibody is specific to a protein of 57 kDa known as p57Kip2 a cell cycle regulatory mitotic inhibitor. This antibody does not cross-react with p27Kip1. p57Kip2 is a potent tight-binding inhibitor of several G1 cyclin complexes and a negative regulator of cell proliferation.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-p57Kip2 Antibody Clone: 57P06
Host and Isotype: Mouse IgG2b, kappa
Recommended Positive Control Sample: Colon carcinoma
Cellular Localization of Antibody 57P06 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with mouse recombinant p57Kip2protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

p57Kip2 Information for Pathologists

Summary:

Also called CDKN1C. First described in 1995 (Genes Dev 1995;9:650). Strongly paternally imprinted gene on 11p15.5, expressed predominantly from the maternal allele in most tissues. Pathophysiology Genetic imprinting: differential DNA methylation of maternal and paternal alleles leading to allele specific expression.

Common Uses By Pathologists:

Differentiate complete hydatidiform mole (no nuclear DNA of maternal origin ? p57kip2 underexpressed) vs. partial hydatidiform mole or spontaneous abortion (p57+, Am J Surg Pathol 2001;25:1225, Am J Clin Pathol 2010;133:196), but molecular genotyping may be helpful because complete moles are rarely p57+ (Am J Surg Pathol 2009;33:1409) and partial moles are rarely p57- (Am J Surg Pathol 2011;35:1586, Am J Surg Pathol 2009;33:805). Identifies maternal 11p15 loss (p57-) with paternal K(ATP) mutation as predominant causative mechanism of focal hyperinsulinism (Mod Pathol 2006;19:122). Microscopic (histologic) images Images hosted on other servers:. Missing Image.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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p57Kip2 [57P06] Antibody (cGMP).