Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)
||Citrate Buffer pH 6.0
||30 min at Room Temperature
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-p57Kip2 Antibody Clone: 57P06
Host and Isotype: Mouse IgG2b, kappa
Recommended Positive Control Sample: Colon carcinoma
Cellular Localization of Antibody 57P06 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Lot specific. Plese contact tech support
Immunogen: BALB/C mice were injected with mouse recombinant p57Kip2protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
p57Kip2 Information for Pathologists
Also called CDKN1C. First described in 1995 (Genes Dev 1995;9:650). Strongly paternally imprinted gene on 11p15.5, expressed predominantly from the maternal allele in most tissues. Pathophysiology Genetic imprinting: differential DNA methylation of maternal and paternal alleles leading to allele specific expression.
Common Uses By Pathologists:
Differentiate complete hydatidiform mole (no nuclear DNA of maternal origin ? p57kip2 underexpressed) vs. partial hydatidiform mole or spontaneous abortion (p57+, Am J Surg Pathol 2001;25:1225, Am J Clin Pathol 2010;133:196), but molecular genotyping may be helpful because complete moles are rarely p57+ (Am J Surg Pathol 2009;33:1409) and partial moles are rarely p57- (Am J Surg Pathol 2011;35:1586, Am J Surg Pathol 2009;33:805). Identifies maternal 11p15 loss (p57-) with paternal K(ATP) mutation as predominant causative mechanism of focal hyperinsulinism (Mod Pathol 2006;19:122). Microscopic (histologic) images Images hosted on other servers:. Missing Image.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.