Western Blot of Recombinant, PC3 and HeLa Cell Lysates using p63 Monoclonal Antibody (TP63/1786).
Western Blot of Recombinant, PC3 and HeLa Cell Lysates using p63 Monoclonal Antibody (TP63/1786).

Anti-p63 Antibody Clone TP63/1786

$ 228.00$ 518.00
Please Select Product Options Below To View The Catalog Number.

SKU: 8626-MSM6
Species: Human
Tested Applications: Western Blot
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
8626-MSM6-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
8626-MSM6-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
8626-MSM6-P2 Size: 200 ug, Tag: Unconjugated $ 518.00
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In Stock.
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SKU: 8626-MSM6-P0
p63 General Information
Alternate Names
Tumor protein p63 transformation-related protein 63, TP63, p63
Molecular Weight
63kDa
Chromosomal Location
3q28
Curated Database and Bioinformatic Data
Gene SymbolTP63
Entrez Gene ID8626
Ensemble Gene IDENSG00000073282
RefSeq Protein Accession(s)NP_003713, XP_016862876, NP_001108451, NP_001108454, XP_005247901, NP_001108450, NP_001316073, NP_001316074, NP_001316077, NP_001108452, XP_005247900, NP_001316075, NP_001316079, XP_011511553, XP_011511554, NP_001108453, NP_001316078, NP_001316893
RefSeq mRNA Accession(s)XM_005247844, NM_001114978, NM_001114980, NM_001329145, NM_001329146, XM_017007387, NM_001114979, XM_005247843, NM_001114982, NM_001329144, NM_001329964, XM_011513252, NM_001329149, XM_011513251, NM_003722, NM_001329148 NM_001114981, NM_001329150
RefSeq Genomic Accession(s)NC_000003, NG_007550, NC_018914,
UniProt ID(s)B7Z8X6, A0A0S2Z4N5, A0A141PNN4, A0A141PNN3, Q9H3D4, C9D7D0, A0A0S2Z4N6
UniGene ID(s)B7Z8X6, A0A0S2Z4N5, A0A141PNN4, A0A141PNN3, Q9H3D4, C9D7D0, A0A0S2Z4N6
HGNC ID(s)15979
Cosmic ID(s)TP63
KEGG Gene ID(s)hsa:8626
PharmGKB ID(s)PA162406776
General Description of p63.
p63 is a homolog of the tumor suppressor p53. It is identified in basal cells in the epithelial layers of a variety of tissues, including epidermis, cervix, urothelium, breast and prostate. p63 was detected in nuclei of the basal epithelium in normal prostate glands ;however, it was not expressed in malignant tumors of the prostate. As a result, p63 has been reported as a useful marker for differentiating benign from malignant lesions in the prostate, particularly when used in combination with markers of high molecular weight cytokeratins and the prostate-specific marker AMACR (P504S). p63 has also been shown to be a sensitive marker for lung squamous cell carcinomas (SqCC), with a sensitivity of ~90%. Specificity for lung SqCC, vs. lung adenocarcinoma (LADC), is approximately 80%. In breast tissue, p63 has been identified in myoepithelial cells of normal ducts.

Human Anti-p63 Antibody Product Attributes

Species: Human
Tested Applications: Western Blot.
Clonality: Monoclonal
Anti-p63 Antibody Clone: TP63/1786
Clone TP63/1786 Host and Isotype: Mouse IgG2b kappa
Anti-Human p63 Positive Control Sample: HEK293 cells or Prostate Carcinoma or Lung or bladder squamous cell carcinoma
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human p63 protein fragment (aa3-106)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

p63 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TP63 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in p63 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, glandular cells in the epididymis, squamous epithelial cells in the esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. More moderate antibody staining intensity was present in myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, glandular cells in the epididymis, squamous epithelial cells in the esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. Low, but measureable presence of p63 could be seen inglandular cells in the breast, cervix, uterine. We were unable to detect p63 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of p63 expression as measured by anti-p63 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - ++ - - - +++ - ++ - - + - - - +++ - + - +++
TP63 Variability + ++ ++ ++ ++ + + ++ ++ + ++ ++ + + + + + ++ ++ +

Limitations and Warranty

enQuire Bio's p63 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-p63 Antibody Clone TP63/1786

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