Human Anti-p63 Antibody Product Attributes
Species: Human
Tested Applications: Western Blot.
Clonality: Monoclonal
Anti-p63 Antibody Clone: TP63/1786
Clone TP63/1786 Host and Isotype: Mouse IgG2b kappa
Anti-Human p63 Positive Control Sample: HEK293 cells or Prostate Carcinoma or Lung or bladder squamous cell carcinoma
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human p63 protein fragment (aa3-106)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
p63 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-TP63 antibody staining is expected to be primarily localized to the nucleoplasm.Observed Antibody Staining Data By Tissue Type:
Variations in p63 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, glandular cells in the epididymis, squamous epithelial cells in the esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. More moderate antibody staining intensity was present in myoepithelial cells in the breast, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, glandular cells in the epididymis, squamous epithelial cells in the esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, Langerhans in skin, melanocytes in skin, epidermal cells in the skin, squamous epithelial cells in the tonsil, urothelial cells in the urinary bladder and squamous epithelial cells in the vagina. Low, but measureable presence of p63 could be seen inglandular cells in the breast, cervix, uterine. We were unable to detect p63 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of p63 expression as measured by anti-p63 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | + | - | ++ | - | - | - | +++ | - | ++ | - | - | + | - | - | - | +++ | - | + | - | +++ |
| TP63 Variability | + | ++ | ++ | ++ | ++ | + | + | ++ | ++ | + | ++ | ++ | + | + | + | + | + | ++ | ++ | + |
Limitations and Warranty
enQuire Bio's p63 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
There are no reviews yet.