Immunohistochemical staining of Pax-8  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Pax-8 [4H7B3] Antibody (cGMP).

$ 375.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD175
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD175-6ml Size: 6 ml, Format: Predilute
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Datasheets and Documentation
Product Datasheet
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SKU: QIVD175-6ml
Pax-8 General Information
Alternate Names
Molecular Weight
48.2 kDa
Chromosomal Location
q14.1 [chr: 2] [chr_start: 113215997] [chr_end: 113278921] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolPAX8
Entrez Gene ID7849
RefSeq Protein Accession(s)NP_039247; NP_039246; NP_054698; NP_003457
RefSeq mRNA Accession(s)NM_013951; NM_013992; NM_013953; NM_003466; NM_013952
RefSeq Genomic Accession(s)NG_012384; NC_000002
UniProt ID(s)Q06710
PharmGKB ID(s)PA32962
KEGG Gene ID(s)hsa:7849
Associated Diseases (KEGG IDs)Hypothyroidism, congenital, non-goitrous, 2 (CHNG2) [MIM:218700]: A disease characterized by thyroid dysgenesis, the most frequent cause of congenital hypothyroidism, accounting for 85% of case. The thyroid gland can be completely absent (athyreosis), ectopically located and/or severely hypoplastic. Ectopic thyroid gland is the most frequent malformation, with thyroid tissue being found most often at the base of the tongue. {ECO:0000269|PubMed:11232006, ECO:0000269|PubMed:11502839, ECO:0000269|PubMed:9590296}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of Pax-8 .
PAX 8 is expressed in a high percentage of renal cell carcinomas and ovarian cancers. This mouse monoclonal PAX 8 antibody [4H7B3] has been designed to target restricted epitopes, and exhibits higher specificity and provides sharper staining than the PAX 8 rabbit polyclonal antibody. Unlike the polyclonal PAX 8, this mouse monoclonal antibody does not stain B-cells, and does not recognize epitopes of pancreatic origin and neuroendocrine cells in stomach and colon; thus providing superior specificity. The expression of the mouse monoclonal PAX 8 targets antigens found in normal kidney, thyroid and cervix, but not normal ovary. PAX 8 stains nuclei exclusively and performs well in formalin-fixed paraffin-embedded tissues.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) Only Available as Predilute
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Pax-8 Antibody Clone: 4H7B3
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Normal kidney, renal cell, or serous ovarian carcinomas
Cellular Localization of Antibody 4H7B3 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: ag0306
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Pax-8 Information for Pathologists

Summary:

Also called Paired box 8. Discovered in 1990 (Development 1990;110:643). Paired domains are composed of two helix-turn-helix subdomains. The N-terminal subdomain and the C-terminal subdomain, both of which can bind DNA independently (Cell 1995;80:639). Structurally similar to PAX2 and PAX5.

Common Uses By Pathologists:

Differentiate PAX8+ primary or metastatic tumors (kidney, Müllerian [but not mucinous, Am J Surg Pathol 2011;35:1837], thymic, thyroid) from PAX8- tumors (adrenal, breast, GI [most], lung, prostate, Am J Surg Pathol 2011;35:816). Differentiate prostatic mesonephric remnant hyperplasia (PAX8+) from prostatic adenocarcinoma (PAX8-, Am J Surg Pathol 2011;35:1054). Differentiate metastatic clear cell renal cell carcinoma (PAX2+ or PAX8+ / inhibinA neg) from hemangioblastoma (PAX2-, PAX8-, inhibin A+, Am J Surg Pathol 2011;35:262). Differentiate endosalpingiosis in axillary lymph nodes (PAX8+ / WT1+) from metastatic breast carcinoma (PAX8-, WT1-, Am J Surg Pathol 2010;34:1211). Differentiate renal collecting duct carcinoma (PAX8+ / p63-) from urothelial carcinoma of upper urinary tract (PAX8- / p63+, Am J Surg Pathol 2010;34:965).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Pax-8 [4H7B3] Antibody (cGMP).