Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with PAX6 Monoclonal Antibody (SPM612).
Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with PAX6 Monoclonal Antibody (SPM612).

Anti-PAX6 Antibody Clone SPM612

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 5080-MSM2X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5080-MSM2X-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: 5080-MSM2X-P1
PAX6 General Information
Alternate Names
Paired box protein Pax-6 aniridia type II protein, PAX6
Molecular Weight
47kDa
Chromosomal Location
11p13
Curated Database and Bioinformatic Data
Gene SymbolPAX6
Entrez Gene ID5080
Ensemble Gene IDENSG00000007372
RefSeq Protein Accession(s)NP_001121084, NP_001595, NP_001245394, NP_001297087, NP_001297088, NP_001245391, NP_001245392, NP_001297089, NP_001297090, NP_001245393, NP_000271
RefSeq mRNA Accession(s)NM_001127612, NM_001258462, NM_001258463, NM_001310160, NM_001310161, NM_001258465, NM_000280, NM_001310158, NM_001604 NM_001310159, NM_001258464
RefSeq Genomic Accession(s)NG_008679, NC_000011, NC_018922
UniProt ID(s)A0A1W2PRA8, P26367, F1T0F8, Q66SS1, D1KF47
UniGene ID(s)A0A1W2PRA8, P26367, F1T0F8, Q66SS1, D1KF47
HGNC ID(s)8620
Cosmic ID(s)PAX6
KEGG Gene ID(s)hsa:5080
PharmGKB ID(s)PA32960
General Description of PAX6.
Pax genes contain paired domains with strong homology to genes in Drosophila, which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain, the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain.

Human Anti-PAX6 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-PAX6 Antibody Clone: SPM612
Clone SPM612 Host and Isotype: Mouse IgG1 kappa
Anti-Human PAX6 Positive Control Sample: Pancreas, Cerebellum or Stomach
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (N-terminus; aa 1-300) of human PAX6 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

PAX6 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-PAX6 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in PAX6 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in cells in the granular layer in cerebellum and islets of Langerhans in pancreas. More moderate antibody staining intensity was present in cells in the granular layer in cerebellum and islets of Langerhans in pancreas. Low, but measureable presence of PAX6 could be seen inglandular cells in the appendix, colon, small intestine, stomach. We were unable to detect PAX6 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of PAX6 expression as measured by anti-PAX6 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
PAX6 Variability + ++ ++ + + ++ + + + + + + + + + ++ + + + +

Limitations and Warranty

enQuire Bio's PAX6 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-PAX6 Antibody Clone SPM612”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   1825 Lawrence St, Ste 115   Denver, CO 80202
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-PAX6 Antibody Clone SPM612

https://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-5080-MSM2X-P1-anti-PAX6-antibody-300x225.jpeg