![Analysis of Mass Spec data (dashed-line) of fractions stained with PCNA MS-QAVA™ monoclonal antibody [Clone: PC10] (solid-line), reveals that less than 3.7% of signal is attributable to non-specific binding of anti-PCNA [Clone PC10] to targets other than PCNA protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-5111-MSM1-P1-anti-PCNA-antibody.png "PCNA Antibody Mass Spec Validation Data")
Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, and Yeast (S pombe and S cerevisiae) Anti-PCNA Antibody Product Attributes
Species: Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, and Yeast (S pombe and S cerevisiae)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVAâ¢. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-PCNA Antibody Clone: PC10
Clone PC10 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, and Yeast (S pombe and S cerevisiae) PCNA Positive Control Sample: Tonsil or reactive lymph node
Cellular Localization of Antibody Predominantly nuclear, some cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Rat PCNA/Protein A fusion protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
PCNA Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Variations in PCNA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the appendix, lymphoid tissue in appendix, hematopoietic cells in the bone marrow, glandular cells in the colon, germinal center cells in the lymph node, glandular cells in the small intestine and germinal center cells in the tonsil. More moderate antibody staining intensity was present in glandular cells in the appendix, lymphoid tissue in appendix, hematopoietic cells in the bone marrow, glandular cells in the colon, germinal center cells in the lymph node, glandular cells in the small intestine and germinal center cells in the tonsil. Low, but measureable presence of PCNA could be seen inglandular cells in the adrenal gland, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, myocytes in heart muscle, macrophages in lung, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland, trophoblastic cells in the placenta, myocytes in skeletal muscle, Langerhans in skin, smooth muscle cells in the smooth muscle, cells in the red pulp in spleen and glandular cells in the stomach. We were unable to detect PCNA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Type:
Tissues from cancer patients, for instance, have their own distinct pattern of PCNA expression as measured by anti-PCNA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | +++ | ++ | +++ | +++ | ++ | - | +++ | ++ | +++ | ++ | +++ | ++ | +++ | + | ++ | +++ | ++ | ++ | + | +++ |
| PCNA Variability | ++ | ++ | + | ++ | +++ | ++ | ++ | +++ | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
Limitations and Warranty
This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
![Analysis of Mass Spec data (dashed-line) of fractions stained with PCNA MS-QAVA™ monoclonal antibody [Clone: PC10] (solid-line), reveals that less than 3.7% of signal is attributable to non-specific binding of anti-PCNA [Clone PC10] to targets other than PCNA protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.](https://cdn-enquirebio.pressidium.com/wp-content/uploads/2017/10/enQuire-Bio-5111-MSM1-P1-anti-PCNA-antibody-178x178.png)
There are no reviews yet.