Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (SPM35)
Formalin-fixed, paraffin-embedded human Tonsil stained with PCNA Monoclonal Antibody (SPM35)

Anti-PCNA Antibody Clone SPM350

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 5111-MSM1X
Species: Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, and Yeast (S pombe & S cerevisiae)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5111-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 5111-MSM1X-P1
PCNA General Information
Alternate Names
Proliferating cell nuclear antigen, PCNA
Molecular Weight
36kDa
Chromosomal Location
20p12.3
Curated Database and Bioinformatic Data
Gene SymbolPCNA
Entrez Gene ID5111
Ensemble Gene IDENSG00000132646
RefSeq Protein Accession(s)NP_872590, NP_002583
RefSeq mRNA Accession(s)NM_002592 NM_182649
RefSeq Genomic Accession(s)NC_000020, NG_047066, NC_018931
UniProt ID(s)P12004
UniGene ID(s)P12004
HGNC ID(s)8729
Cosmic ID(s)PCNA
KEGG Gene ID(s)hsa:5111
PharmGKB ID(s)PA263
General Description of PCNA.
Recognizes a non-histone protein of 36kDa, which is identified as proliferating cell nuclear antigen (PCNA). It is also known as cyclin or polymerase delta auxiliary protein. Elevated expression of PCNA/cyclin has been shown in the nucleus during late G1 phase immediately before the onset of DNA synthesis, becoming maximal during S-phase, declining during G2, M phases. This MAb is excellent for multiple applications.

Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, and Yeast (S pombe & S cerevisiae) Anti-PCNA Antibody Product Attributes

Species: Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, and Yeast (S pombe & S cerevisiae)
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-PCNA Antibody Clone: SPM350
Clone SPM350 Host and Isotype: Mouse IgG2a kappa
Anti-Human, Monkey, Pig, Mouse, Rat, Chicken, Zebrafish, Drosophila melanogaster, and Yeast (S pombe & S cerevisiae) PCNA Positive Control Sample: Tonsil or reactive lymph node
Cellular Localization of Antibody Predominantly nuclear, some cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Rat PCNA/Protein A fusion protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

PCNA Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-PCNA antibody staining is expected to be primarily localized to the nuclear bodies and nucleoplasm. There is variability in either the signal strength or the localization of signal in nuclear bodies and nucleoplasm from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in PCNA antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the appendix, lymphoid tissue in appendix, hematopoietic cells in the bone marrow, glandular cells in the colon, germinal center cells in the lymph node, glandular cells in the small intestine and germinal center cells in the tonsil. More moderate antibody staining intensity was present in glandular cells in the appendix, lymphoid tissue in appendix, hematopoietic cells in the bone marrow, glandular cells in the colon, germinal center cells in the lymph node, glandular cells in the small intestine and germinal center cells in the tonsil. Low, but measureable presence of PCNA could be seen inglandular cells in the adrenal gland, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, myocytes in heart muscle, macrophages in lung, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland, trophoblastic cells in the placenta, myocytes in skeletal muscle, Langerhans in skin, smooth muscle cells in the smooth muscle, cells in the red pulp in spleen and glandular cells in the stomach. We were unable to detect PCNA in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of PCNA expression as measured by anti-PCNA antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity +++ ++ +++ +++ ++ - +++ ++ +++ ++ +++ ++ +++ + ++ +++ ++ ++ + +++
PCNA Variability ++ ++ + ++ +++ ++ ++ +++ + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's PCNA Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-PCNA Antibody Clone SPM350

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