Human Anti-PD1 / PDCD1 / CD279 Antibody Product Attributes
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-PD1 / PDCD1 / CD279 Antibody Clone: SPM597
Clone SPM597 Host and Isotype: Mouse IgG1 kappa
Anti-Human PD1 / PDCD1 / CD279 Positive Control Sample: TY cells or Tonsil.
Cellular Localization of Antibody Cell Surface & Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human PDCD1 protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
PD1 / PDCD1 / CD279 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in PD1 / PDCD1 / CD279 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in germinal center cells in the tonsil. More moderate antibody staining intensity was present in germinal center cells in the tonsil. Low, but measureable presence of PD1 / PDCD1 / CD279 could be seen in cells in the red pulp in spleen. We were unable to detect PD1 / PDCD1 / CD279 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's PD1 / PDCD1 / CD279 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.