Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Pgp9.5 Monoclonal Antibody (UCHL1/775).
Formalin-fixed, paraffin-embedded Rat Cerebellum stained with Pgp9.5 Monoclonal Antibody (UCHL1/775).

Anti-PGP9.5 / UchL1 Antibody Clone UCHL1/775

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7345-MSM3
Species: Human, Rat, and Broad Species Reactivity
Tested Applications: Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7345-MSM3-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 7345-MSM3-P1
PGP9.5 / UchL1 General Information
Alternate Names
Ubiquitin carboxy-terminal hydrolase L1, UCHL1
Molecular Weight
20-30kDa
Chromosomal Location
4p13
Curated Database and Bioinformatic Data
Gene SymbolUCHL1
Entrez Gene ID7345
Ensemble Gene IDENSG00000154277
RefSeq Protein Accession(s)NP_004172
RefSeq mRNA Accession(s)NM_004181,
RefSeq Genomic Accession(s)NC_018915, NG_012931, NC_000004
UniProt ID(s)V9HW74, P09936
UniGene ID(s)V9HW74, P09936
HGNC ID(s)12513
Cosmic ID(s)UCHL1
KEGG Gene ID(s)hsa:7345
PharmGKB ID(s)PA37160
General Description of PGP9.5 / UchL1.
This MAb reacts with a protein of 20-30kDa, identified as PGP9.5, also known as ubiquitin carboxyl-terminal hydrolase-1 (UchL1). Initially, PGP9.5 expression in normal tissues was reported in neurons, neuroendocrine cells but later it was found in distal renal tubular epithelium, spermatogonia, Leydig cells, oocytes, melanocytes, prostatic secretory epithelium, ejaculatory duct cells, epididymis, mammary epithelial cells, Merkel cells,, dermal fibroblasts. Furthermore, immunostaining for PGP9.5 has been shown in a wide variety of mesenchymal neoplasms as well. A mutation in PGP9.5 gene is believed to cause a form of Parkinson's disease.

Human, Rat, and Broad Species Reactivity Anti-PGP9.5 / UchL1 Antibody Product Attributes

Species: Human, Rat, and Broad Species Reactivity
Tested Applications: Western Blot, Immunohistochemistry (IHC).
Application Notes: Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-PGP9.5 / UchL1 Antibody Clone: UCHL1/775
Clone UCHL1/775 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Rat, and Broad Species Reactivity PGP9.5 / UchL1 Positive Control Sample: Cerebellum
Cellular Localization of Antibody Cytoplasmic. Endoplasmic Reticulum membrane
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human UCHL1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

PGP9.5 / UchL1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-UCHL1 antibody staining is expected to be primarily localized to the cytosol and nucleoplasm. There is variability in either the signal strength or the localization of signal in cytosol from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in PGP9.5 / UchL1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in neuronal cells in the caudate nucleus, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, neuronal cells in the cerebral cortex, peripheral nerve/ganglion in colon, neuronal cells in the hippocampus, cells in the tubules in kidney and islets of Langerhans in pancreas. More moderate antibody staining intensity was present in neuronal cells in the caudate nucleus, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, neuronal cells in the cerebral cortex, peripheral nerve/ganglion in colon, neuronal cells in the hippocampus, cells in the tubules in kidney and islets of Langerhans in pancreas. Low, but measureable presence of PGP9.5 / UchL1 could be seen in cells in the seminiferous ducts in testis and Leydig cells in the testis. We were unable to detect PGP9.5 / UchL1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of PGP9.5 / UchL1 expression as measured by anti-PGP9.5 / UchL1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - + - - - + - - - - - - - - - - - - - -
UCHL1 Variability + +++ + + + ++ + + ++ + + + + + + + + ++ + +

Limitations and Warranty

enQuire Bio's PGP9.5 / UchL1 Anti-Human, Rat Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-PGP9.5 / UchL1 Antibody Clone UCHL1/775

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