Immunohistochemical staining of Phosphohistone H3  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Phosphohistone H3 [Polyclonal] Antibody (cGMP).

$ 158.00$ 618.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD177
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Undetermined

Product NumberDescriptionPrice
QIVD177-0.1ml Size: 0.1 ml, Format: Concentrate $ 158.00
QIVD177-0.5ml Size: 0.5 ml, Format: Concentrate $ 288.00
QIVD177-1ml Size: 1 ml, Format: Concentrate $ 478.00
QIVD177-6ml Size: 6 ml, Format: Predilute $ 368.00
QIVD177-25ml Size: 25 ml, Format: Predilute $ 618.00
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Datasheets and Documentation
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SKU: QIVD177-0.1ml
Phosphohistone H3 General Information
Alternate Names
Molecular Weight
15.4 kDa
Curated Database and Bioinformatic Data
Gene SymbolHHT2; HHT1
UniProt ID(s)P69150
General Description of Phosphohistone H3 .
Phosphohistone H3 (PHH3) is a core histone protein, which together with other histones, forms the major protein constituents of the chromatin in eukaryotic cells. In mammalian cells, phosphohistone H3 is negligible during interphase but reaches a maximum for chromatin condensation during mitosis.Immunohistochemical studies showed anti-PHH3 specifically detected the core protein histone H3 only when phosphorylated at serine 10 or serine 28. Studies have also revealed no phosphorylation on the histone H3 during apoptosis.PHH3 can serve as a mitotic marker to separate mitotic figures from apoptotic bodies and karyorrhectic debris, which may be a very useful tool in diagnosis of tumor grades, especially in CNS, skin, gyn., soft tissue, and GIST.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Polyclonal
Anti-Phosphohistone H3 Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Melanoma
Cellular Localization of Antibody Polyclonal Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Phosphohistone protein n isolated from human tissue.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Phosphohistone H3 Information for Pathologists

Summary:

Core histone protein that is major constituent of chromatin; marker of cells in late G2 and M phase. Interpretation Nuclear stain. Count phosphohistone H3+ objects (nuclei and mitoses) in 10 adjacent fields with 40x objective, in invasive epithelial areas with highest phosphohistone H3 staining. Ignore nuclei with fine granular staining.

Common Uses By Pathologists:

Identify mitotic activity in tumor cells; use for counting mitotic figures. Strong prognostic value in lymph node negative breast cancer patients Mod Pathol 2007;20:1307, Mod Pathol 2011;24:502). Strong prognostic value (Cell Oncol 2009;31:261). Prognostic value in meningiomas (Am J Clin Pathol 2007;128:118, Am J Surg Pathol 2004;28:1532). Microscopic (histologic) images

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Phosphohistone H3 [Polyclonal] Antibody (cGMP).