All,species Anti-Phosphotyrosine Antibody Clone PY20
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Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b
Mass Spec Validated?: Not MS Validated
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunoprecipitation (1-2ug of antibody/500ug protein Lysate), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Anti-Phosphotyrosine Antibody Clone: PY20
Clone PY20 Host and Isotype: Mouse IgG2b
Anti-All,species Phosphotyrosine Positive Control Sample: MCF-7, MDA-231, T47-D cells or breast carcinoma
Cellular Localization of Antibody
Depends upon the location of phosphorylated target
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Phosphotyrosine conjugated to KLH
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
enQuire Bio's Phosphotyrosine Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.