Human Anti-Placental Alkaline Phosphatase Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Placental Alkaline Phosphatase Antibody Clone: SPM593
Clone SPM593 Host and Isotype: Mouse IgG2b kappa
Anti-Human Placental Alkaline Phosphatase Positive Control Sample: HepG2 cells. Placenta or seminoma
Cellular Localization of Antibody Cytoplasmic, cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human ALPP protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Placental Alkaline Phosphatase Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-Alpp antibody staining is expected to be primarily localized to the plasma membrane.Observed Antibody Staining Data By Tissue Type:
Variations in Placental Alkaline Phosphatase antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in trophoblastic cells in the placenta. More moderate antibody staining intensity was present in trophoblastic cells in the placenta. Low, but measureable presence of Placental Alkaline Phosphatase could be seen inglandular cells in the cervix, uterine, squamous epithelial cells in the cervix and uterine. We were unable to detect Placental Alkaline Phosphatase in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Placental Alkaline Phosphatase expression as measured by anti-Placental Alkaline Phosphatase antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | - | - | + | - | - | - | - | - | - | + | - | - | - | - | - | +++ | - | - |
| ALPP Variability | + | + | ++ | + | ++ | + | + | + | + | + | + | ++ | + | + | + | + | ++ | + | + | + |
Limitations and Warranty
enQuire Bio's Placental Alkaline Phosphatase Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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