Formalin-fixed, paraffin-embedded human Placenta stained with PLAP Monoclonal Antibody (SPM593).
Formalin-fixed, paraffin-embedded human Placenta stained with PLAP Monoclonal Antibody (SPM593).

Anti-Placental Alkaline Phosphatase Antibody Clone SPM593

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 250-MSM1X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
250-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 250-MSM1X-P1
Placental Alkaline Phosphatase General Information
Alternate Names
Alkaline phosphatase, placental type, placental alkaline phosphatase, PLAP,
Molecular Weight
70kDa
Chromosomal Location
2q37.1
Curated Database and Bioinformatic Data
Gene SymbolALPP
Entrez Gene ID250
Ensemble Gene IDENSG00000163283
RefSeq Protein Accession(s)NP_001623
RefSeq mRNA Accession(s)NM_001632
RefSeq Genomic Accession(s)NC_018913, NC_000002, NG_012189
UniProt ID(s)B2R7C7, P05187
UniGene ID(s)B2R7C7, P05187
HGNC ID(s)439
Cosmic ID(s)ALPP
KEGG Gene ID(s)hsa:250
PharmGKB ID(s)PA24730
General Description of Placental Alkaline Phosphatase.
Reacts with a 70kDa membrane-bound isozyme (Regan, Nagao type) of Placental Alkaline Phosphatase (PLAP) occurring in the placenta during the 3rd trimester of gestation. It is highly specific for PLAP, shows no cross-reaction with other isozymes of alkaline phosphatase. Anti-PLAP reacts with germ cell tumors, can discriminate between these, other neoplasms. Somatic neoplasms e.g. breast, gastrointestinal, prostatic,, urinary cancers may also immunoreact with antibodies to PLAP. Anti-PLAP positivity in conjunction with anti-keratin negativity favors seminoma over carcinoma. Germ cell tumors are usually anti-keratin positive, but they regularly fail to stain with anti-EMA, whereas most carcinomas stain with anti-EMA. Anti-PLAP has been useful in the diagnosis of gestational trophoblastic disease.

Human Anti-Placental Alkaline Phosphatase Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.25-0.5ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Placental Alkaline Phosphatase Antibody Clone: SPM593
Clone SPM593 Host and Isotype: Mouse IgG2b kappa
Anti-Human Placental Alkaline Phosphatase Positive Control Sample: HepG2 cells. Placenta or seminoma
Cellular Localization of Antibody Cytoplasmic, cell surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human ALPP protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Placental Alkaline Phosphatase Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-Alpp antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Placental Alkaline Phosphatase antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in trophoblastic cells in the placenta. More moderate antibody staining intensity was present in trophoblastic cells in the placenta. Low, but measureable presence of Placental Alkaline Phosphatase could be seen inglandular cells in the cervix, uterine, squamous epithelial cells in the cervix and uterine. We were unable to detect Placental Alkaline Phosphatase in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Placental Alkaline Phosphatase expression as measured by anti-Placental Alkaline Phosphatase antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - + - - - - - - + - - - - - +++ - -
ALPP Variability + + ++ + ++ + + + + + + ++ + + + + ++ + + +

Limitations and Warranty

enQuire Bio's Placental Alkaline Phosphatase Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Placental Alkaline Phosphatase Antibody Clone SPM593

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