Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)||1:25-1:50|
|Pretreatment||EDTA Buffer pH 8.0|
|Incubation Parameters||30 min at Room Temperature|
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-Podoplanin Antibody Clone: D2-40
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody D2-40 Staining: Transmembrane and Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Dysgerminoma Tissue
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Podoplanin Information for Pathologists
Summary:38 kDa membrane mucoprotein originally detected on surface of rat podocytes. Antibody D2-40 reacts to an O-linked, 40 kDa sialoglycoprotein found on lymphatic endothelium, fetal testis and on the surface of testicular germ cell tumors (also other cells as indicated below). Uses by pathologists Identify lymphatic endothelium to determine presence of lymphatics in various conditions, as well as origin of vascular tumors (J Histochem Cytochem 2009;57:643). Identify lymphatic endothelium to determine lymphatic invasion (Ann Diagn Pathol 2009;13:168).
Common Uses By Pathologists:Identify lymphatic endothelium to determine presence of lymphatics in various conditions, as well as origin of vascular tumors (J Histochem Cytochem 2009;57:643). Identify lymphatic endothelium to determine lymphatic invasion (Ann Diagn Pathol 2009;13:168). Differentiate mesothelioma (positive, even in effusions, Diagn Cytopathol 2007;35:342) from adenocarcinoma (negative, Cancer 2007;109:933). Note: prognostic significance of lymphatic invasion using D2-40 versus H&E only is unclear:. Not clearly significant - breast carcinoma (Mod Pathol 2009;22:216).
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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