Immunohistochemical staining of Prostate Specific Antigen  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Prostate Specific Antigen [Polyclonal] Antibody (cGMP).

$ 128.00$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD65
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Rabbit Undetermined

Product NumberDescriptionPrice
QIVD65-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD65-0.5ml Size: 0.5 ml, Format: Concentrate $ 178.00
QIVD65-1ml Size: 1 ml, Format: Concentrate $ 298.00
QIVD65-6ml Size: 6 ml, Format: Predilute $ 128.00
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Datasheets and Documentation
Product Datasheet
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SKU: QIVD65-0.1ml
Prostate Specific Antigen General Information
Alternate Names
Molecular Weight
28.7 kDa
Chromosomal Location
q13.33 [chr: 19] [chr_start: 50854915] [chr_end: 50860764] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolKLK3
Entrez Gene ID354
RefSeq Protein Accession(s)NP_001025219; NP_001025218; NP_001639
RefSeq mRNA Accession(s)NM_001648; NM_001030047; NM_001030050; NM_001030048; NM_001030049
RefSeq Genomic Accession(s)NG_011653; NC_000019;
UniProt ID(s)P07288
PharmGKB ID(s)PA164741810
KEGG Gene ID(s)hsa:354
General Description of Prostate Specific Antigen .
This antibody reacts with PSA. The staining was observed in the prostatic ductal epithelium of normal and neoplastic prostate. No staining with any other cell type has been observed.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment No Pretreatment Required
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Polyclonal
Anti-Prostate Specific Antigen Antibody Clone: Polyclonal
Host and Isotype: Rabbit Undetermined
Recommended Positive Control Sample: Prostate carcinoma
Cellular Localization of Antibody Polyclonal Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Prostate specific antigen isolated from human seminal plasma.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Prostate Specific Antigen Information for Pathologists

Summary:

Kallikrein related serine protease produced by secretory epithelium, drains into ductal system. Cleaves and liquefies seminal coagulum formed after ejaculation. Clinical features Serum PSA > 4 seen in 80% with histologically documented cancer but also in 25 - 30% with nodular hyperplasia, prostatitis, infarcts, prostatic massage, cystoscopy. Elevated in 2 of 18 post-race marathon runners (Arch Pathol Lab Med 2003;127:345).

Common Uses By Pathologists:

Glycoprotein detected in benign and malignant prostate tissue. Identifies prostatic origin of most metastatic tumors, differentiates between prostatic and urothelial carcinomas. Non-prostate tumors are usually weak / negative for PSA / PAP. PSA is more sensitive (intense, diffuse staining) and specific than PAP. Microscopic (histologic) images

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Prostate Specific Antigen [Polyclonal] Antibody (cGMP).