Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with pS2 Monoclonal Antibody (SPM573).
Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with pS2 Monoclonal Antibody (SPM573).

Anti-pS2 / pNR-2 / Trefoil Factor 1 Antibody Clone SPM573

$ 488.00
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SKU: 7031-MSM1X
Species: Human and Cynomolgus Monkey
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7031-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 7031-MSM1X-P1
pS2 / pNR-2 / Trefoil Factor 1 General Information
Alternate Names
Trefoil factor 1, TFF1
Molecular Weight
6.5kDa
Chromosomal Location
16p11.2
Curated Database and Bioinformatic Data
Gene SymbolTFF1
Entrez Gene ID7031
Ensemble Gene IDENSG00000160182
RefSeq Protein Accession(s)NP_003216
RefSeq mRNA Accession(s)NM_003225
RefSeq Genomic Accession(s)NC_000021, NC_018932
UniProt ID(s)P04155
UniGene ID(s)P04155
HGNC ID(s)11755
Cosmic ID(s)TFF1
KEGG Gene ID(s)hsa:7031
PharmGKB ID(s)PA36470
General Description of pS2 / pNR-2 / Trefoil Factor 1.
It recognizes a polypeptide of 6.5kDa, identified as pS2 estrogen-regulated protein. Its epitope is localized between aa57-84 of human pS2 protein. pS2 is a trefoil peptide. Trefoil peptides are protease resistant molecules secreted throughout the gut that play a role in mucosal healing. These peptides contain three intra-chain disulfide bonds, forming the trefoil motif, or P-domain. pS2 is known to form dimers, this dimerization is thought to play a role in its protective, healing properties. About 60% of breast carcinomas are positive for pS2. Staining is cytoplasmic, often with localization to the Golgi apparatus. pS2 is shown to be localized in normal stomach mucosa, gastric fluid, goblet cells in the colon, small intestine,, in ulcerations of the gastrointestinal tract. Several studies have shown that pS2 is primarily expressed in estrogen receptor-positive breast tumors, it may define a subset of estrogen-dependent tumors that displays an increased likelihood of response to endocrine therapy.

Human and Cynomolgus Monkey Anti-pS2 / pNR-2 / Trefoil Factor 1 Antibody Product Attributes

Species: Human and Cynomolgus Monkey
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-pS2 / pNR-2 / Trefoil Factor 1 Antibody Clone: SPM573
Clone SPM573 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Cynomolgus Monkey pS2 / pNR-2 / Trefoil Factor 1 Positive Control Sample: Spent medium of MCF-7 cells, treated with estrogen. Normal stomach. About 60% of breast carcinomas are positive for pS2, especially those that are also positive for estrogen,/or progesterone receptor.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Synthetic peptide of 28 amino acid residues corresponding to CFDDTVRGVPWCFYPNTIDVPPEEECEF (aa57-84) from the C-terminus of human pS2.
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

pS2 / pNR-2 / Trefoil Factor 1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in pS2 / pNR-2 / Trefoil Factor 1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the stomach. More moderate antibody staining intensity was present in glandular cells in the stomach. Low, but measureable presence of pS2 / pNR-2 / Trefoil Factor 1 could be seen inglandular cells in the appendix, colon, duodenum, gallbladder, rectum, small intestine. We were unable to detect pS2 / pNR-2 / Trefoil Factor 1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of pS2 / pNR-2 / Trefoil Factor 1 expression as measured by anti-pS2 / pNR-2 / Trefoil Factor 1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
TFF1 Variability ++ ++ + + + + + + + + + ++ ++ + + + + + + +

Limitations and Warranty

enQuire Bio's pS2 / pNR-2 / Trefoil Factor 1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-pS2 / pNR-2 / Trefoil Factor 1 Antibody Clone SPM573

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