Human and Mouse Anti-Rb1 Antibody Product Attributes
Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVAâ¢. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-Rb1 Antibody Clone: 1F8
Clone 1F8 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse Rb1 Positive Control Sample: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.
Cellular Localization of Antibody <1F8 Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Rb1 protein fragment of 283 amino acid residues
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Rb1 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Variations in Rb1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the adrenal gland and breast, Purkinje cells in the cerebellum, glandular cells in the cervix, uterine, non-germinal center cells in the lymph node, decidual cells in the placenta, cells in the inner nuclear layer in retina, cells in the photoreceptor layer in retina, ganglion cells in the retina, melanocytes in skin, glandular cells in the thyroid gland, non-germinal center cells in the tonsil and squamous epithelial cells in the vagina. More moderate antibody staining intensity was present in glandular cells in the adrenal gland and breast, Purkinje cells in the cerebellum, glandular cells in the cervix, uterine, non-germinal center cells in the lymph node, decidual cells in the placenta, cells in the inner nuclear layer in retina, cells in the photoreceptor layer in retina, ganglion cells in the retina, melanocytes in skin, glandular cells in the thyroid gland, non-germinal center cells in the tonsil and squamous epithelial cells in the vagina. Low, but measureable presence of Rb1 could be seen inlymphoid tissue in appendix, adipocytes in breast, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, endothelial cells in the colon, cells in the endometrial stroma in endometrium, glandular cells in the endometrium, islets of Langerhans in pancreas, glandular cells in the prostate and cells in the red pulp in spleen. We were unable to detect Rb1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Type:
Tissues from cancer patients, for instance, have their own distinct pattern of Rb1 expression as measured by anti-Rb1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | ++ | + | - | ++ | + | + | ++ | + | + | + | + | + | + | - | + | + | + | - | + | + |
RB1 Variability | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | +++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
Limitations and Warranty
This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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