Analysis of Mass Spec data (dashed-line) of fractions stained with Rb1 MS-QAVA™ monoclonal antibody [Clone: 1F8] (solid-line), reveals that less than 0.8% of signal is attributable to non-specific binding of anti-Rb1 [Clone: 1F8] to targets other than RB1 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in Jurkat, U202 and HeLa cells.

Anti-Rb1 Antibody Clone 1F8

$ 199.00$ 459.00
Please Select Product Options Below To View The Catalog Number.

SKU: 5925-MSM1
Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: MS-QAVA™ Validated

Product NumberDescriptionPrice
5925-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
5925-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
5925-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 459.00
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: 5925-MSM1-P0
Rb1 General Information
Alternate Names
The retinoblastoma protein, pRb, RB1
Molecular Weight
105kDa
Chromosomal Location
13q14.2
Curated Database and Bioinformatic Data
Gene SymbolRB1
Entrez Gene ID5925
Ensemble Gene IDENSG00000139687
RefSeq Protein Accession(s)XP_011533473, NP_000312
RefSeq mRNA Accession(s)NM_000321, XM_011535171
RefSeq Genomic Accession(s)NG_009009, NC_000013, NC_018924
UniProt ID(s)A0A024RDV3, P06400
UniGene ID(s)A0A024RDV3, P06400
HGNC ID(s)9884
Cosmic ID(s)RB1
KEGG Gene ID(s)hsa:5925
PharmGKB ID(s)PA295
General Description of Rb1.
Recognizes a 105kDa phosphoprotein, identified as retinoblastoma (Rb) gene product. Its epitope is localized between aa 703-772. It shows no cross reaction with p107 or p130. It specifically stains the nuclei of BT-20 cells and primary human foreskin fibroblast (HFF) cells. It does not stain the Rb-negative BT549 cells. It reacts with the hyperphosphorylated as well as the un (under) phosphorylated form of the Rb protein. Retinoblastoma gene product plays a key role in cell cycle control. It has been identified as a tumor suppressor gene whose loss of its function leads to tumor development. It is widely expressed in a variety of human tissues including breast, esophageal, squamous cell and cervical carcinoma.

Human and Mouse Anti-Rb1 Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-Rb1 Antibody Clone: 1F8
Clone 1F8 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse Rb1 Positive Control Sample: HeLa, A431 or BT-20 cells. Breast or Colon carcinoma.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Rb1 protein fragment of 283 amino acid residues
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Rb1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in Rb1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the adrenal gland and breast, Purkinje cells in the cerebellum, glandular cells in the cervix, uterine, non-germinal center cells in the lymph node, decidual cells in the placenta, cells in the inner nuclear layer in retina, cells in the photoreceptor layer in retina, ganglion cells in the retina, melanocytes in skin, glandular cells in the thyroid gland, non-germinal center cells in the tonsil and squamous epithelial cells in the vagina. More moderate antibody staining intensity was present in glandular cells in the adrenal gland and breast, Purkinje cells in the cerebellum, glandular cells in the cervix, uterine, non-germinal center cells in the lymph node, decidual cells in the placenta, cells in the inner nuclear layer in retina, cells in the photoreceptor layer in retina, ganglion cells in the retina, melanocytes in skin, glandular cells in the thyroid gland, non-germinal center cells in the tonsil and squamous epithelial cells in the vagina. Low, but measureable presence of Rb1 could be seen inlymphoid tissue in appendix, adipocytes in breast, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, endothelial cells in the colon, cells in the endometrial stroma in endometrium, glandular cells in the endometrium, islets of Langerhans in pancreas, glandular cells in the prostate and cells in the red pulp in spleen. We were unable to detect Rb1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of Rb1 expression as measured by anti-Rb1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + - ++ + + ++ + + + + + + - + + + - + +
RB1 Variability ++ ++ ++ ++ ++ ++ ++ ++ +++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

There are no reviews yet.

Be the first to review “Anti-Rb1 Antibody Clone 1F8”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Anti-Rb1 Antibody Clone 1F8

https://enquirebio.com/wp-content/uploads/2019/01/Rb1 IHC human Colon (1F8)-300x225.jpghttps://enquirebio.com/wp-content/uploads/2017/10/enQuire-Bio-5925-MSM1-P0-anti-Rb1-antibody-300x271.png