Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with RCC Monoclonal Antibody (66.4.C2).
Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with RCC Monoclonal Antibody (66.4.C2).

Anti-Renal Cell Carcinoma / gp200 Antibody Clone 66.4.C2

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 768-MSM1
Species: Human and Horse
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
768-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 768-MSM1-P1
Renal Cell Carcinoma / gp200 General Information
Alternate Names
Carbonic anhydrase 9, CA9, CAIX
Molecular Weight
55kDa
Chromosomal Location
9p13.3
Curated Database and Bioinformatic Data
Gene SymbolCA9
Entrez Gene ID768
Ensemble Gene IDENSG00000107159
RefSeq Protein Accession(s)NP_001207
RefSeq mRNA Accession(s)XR_428428, NM_001216, XR_001746374
RefSeq Genomic Accession(s)NC_000009, NC_018920
UniProt ID(s)A0A0S2Z3D0, Q16790
UniGene ID(s)A0A0S2Z3D0, Q16790
HGNC ID(s)1383
Cosmic ID(s)CA9
KEGG Gene ID(s)hsa:768
PharmGKB ID(s)PA25998
General Description of Renal Cell Carcinoma / gp200.
Recognizes a glycoprotein of ~200kDa, identified as carbonic anhydrase IX (CAIX/gp200). Its epitope resides in the carbohydrate domain of gp200. It shows no significant cross-reactivity with other carbohydrate determinants, such as the Lewis blood group antigens, epithelial membrane antigen, HMFG,, AB blood group antigens. In normal kidney, gp200 is localized along the brush border of the pars convoluta, pars recta segments of the proximal tubule, as well as focally along the luminal surface of Bowman's capsule adjoining the outgoing proximal tubule. Reportedly, gp200 is expressed by 93% of primary, 84% of metastatic renal cell carcinomas. This MAb may be useful in the investigations of carcinomas of proximal nephrogenic differentiation especially those showing tubular differentiation.

Human and Horse Anti-Renal Cell Carcinoma / gp200 Antibody Product Attributes

Species: Human and Horse
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Renal Cell Carcinoma / gp200 Antibody Clone: 66.4.C2
Clone 66.4.C2 Host and Isotype: Mouse IgG2b kappa
Anti-Human and Horse Renal Cell Carcinoma / gp200 Positive Control Sample: Normal kidney or renal cell carcinoma
Cellular Localization of Antibody Cell surface, cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Microsomal fraction of human renal cortical tissue homogenate
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Renal Cell Carcinoma / gp200 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-CA9 antibody staining is expected to be primarily localized to the plasma membrane and cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in Renal Cell Carcinoma / gp200 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the gallbladder, stomach. More moderate antibody staining intensity was present in glandular cells in the gallbladder, stomach. Low, but measureable presence of Renal Cell Carcinoma / gp200 could be seen in. We were unable to detect Renal Cell Carcinoma / gp200 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Renal Cell Carcinoma / gp200 expression as measured by anti-Renal Cell Carcinoma / gp200 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
CA9 Variability + + + + + + + + + + + + + + ++ + ++ + + ++

Limitations and Warranty

enQuire Bio's Renal Cell Carcinoma / gp200 Anti-Human, Horse Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Renal Cell Carcinoma / gp200 Antibody Clone 66.4.C2

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