Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with RBP1 (SPM442)
Formalin-fixed, paraffin-embedded human Hepatocellular Carcinoma stained with RBP1 (SPM442)

Anti-Retinol Binding Protein-1 Antibody Clone SPM442

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 5947-MSM1X
Species: Human, Monkey, Goat, Rabbit, Rat, and Mouse
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
5947-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 5947-MSM1X-P1
Retinol Binding Protein-1 General Information
Alternate Names
Retinol binding protein 1, cellular RBP1, RBP1
Molecular Weight
21-25kDa
Chromosomal Location
10q23.33
Curated Database and Bioinformatic Data
Gene SymbolRBP1
Entrez Gene ID5947, 5948, 5950
Ensemble Gene IDENSG00000114115, ENSG00000114113, ENSG00000138207
RefSeq Protein Accession(s)NP_001124464, NP_002890, NP_001124465, NP_004155, NP_001310446, NP_001310447, NP_006735
RefSeq mRNA Accession(s)NM_002899, NM_001130993, NM_001130992, NM_004164, NM_001323517, NM_001323518, NM_006744,
RefSeq Genomic Accession(s)NC_018914, NG_047073, NC_000003, NC_000003, NC_018914NC_000010, NC_018921, NG_009104
UniProt ID(s)A0A0A0MQT0, P09455, P50120, Q5VY30, P02753
UniGene ID(s)A0A0A0MQT0, P09455, P50120, Q5VY30, P02753
HGNC ID(s)9919, 9920, 9922
Cosmic ID(s)RBP1, RBP2, RBP4
KEGG Gene ID(s)hsa:5947, hsa:5948, hsa:5950
PharmGKB ID(s)PA34286, PA34287, PA34289
General Description of Retinol Binding Protein-1.
Recognizes a protein of 21kDa-25kDa, identified as retinol binding protein (RBP). Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced, carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin). RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.

Human, Monkey, Goat, Rabbit, Rat, and Mouse Anti-Retinol Binding Protein-1 Antibody Product Attributes

Species: Human, Monkey, Goat, Rabbit, Rat, and Mouse
Tested Applications: Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Retinol Binding Protein-1 Antibody Clone: SPM442
Clone SPM442 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Monkey, Goat, Rabbit, Rat, and Mouse Retinol Binding Protein-1 Positive Control Sample: Liver or placenta
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Retinol binding protein purified from human plasma
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Retinol Binding Protein-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-RBP1 antibody staining is expected to be primarily localized to the cytosol and nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in Retinol Binding Protein-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in Leydig cells in the testis. More moderate antibody staining intensity was present in Leydig cells in the testis. Low, but measureable presence of Retinol Binding Protein-1 could be seen inglandular cells in the breast, glial cells in the caudate nucleus, cells in the molecular layer in cerebellum, glandular cells in the cervix, uterine, cells in the endometrial stroma in endometrium, glandular cells in the gallbladder, glial cells in the hippocampus, cells in the tubules in kidney and cells in the seminiferous ducts in testis. We were unable to detect Retinol Binding Protein-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Retinol Binding Protein-1 expression as measured by anti-Retinol Binding Protein-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - + - - + - - - - - - - - - - - - -
RBP1 Variability + ++ + ++ ++ ++ ++ + ++ + + ++ ++ + + ++ + ++ + ++

Limitations and Warranty

enQuire Bio's Retinol Binding Protein-1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Retinol Binding Protein-1 Antibody Clone SPM442

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