Immunohistochemical staining of RPA/p34  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

RPA/p34 [9H8] Antibody (cGMP).

$ 135.00$ 775.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD193
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1, kappa

Product NumberDescriptionPrice
QIVD193-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD193-0.5ml Size: 0.5 ml, Format: Concentrate $ 465.00
QIVD193-1ml Size: 1 ml, Format: Concentrate $ 775.00
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Datasheets and Documentation
Product Datasheet
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SKU: QIVD193-0.1ml
RPA/p34 General Information
Alternate Names
Molecular Weight
29.2 kDa
Chromosomal Location
p35.3 [chr: 1] [chr_start: 27891524] [chr_end: 27914746] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolRPA2
Entrez Gene ID6118
RefSeq Protein Accession(s)NP_002937; NP_001284487; NP_001273005
RefSeq mRNA Accession(s)NM_002946; XM_024448862; NM_001355128; NM_001286076; NM_001297558; XM_024448864; NM_001355129
RefSeq Genomic Accession(s)NC_000001
UniProt ID(s)P15927
PharmGKB ID(s)PA34652
KEGG Gene ID(s)hsa:6118
General Description of RPA/p34 .
This antibody reacts with a 32-34 kDa protein known as replication protein A (RPA). RPA is involved in DNA replication, repair and recombination. Human RPA is a stable heterotrimer of 70 kDa, 32-34 kDa and 11-14 kDa subunits (RPA70, RPA32 and RPA14 respectively). RPA is required for the SV40 large tumor antigen-catalyzed unwinding for SV40 DNA and stimulates DNA polymerase alpha and δ.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-RPA/p34 Antibody Clone: 9H8
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Tonsil
Cellular Localization of Antibody 9H8 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with purified human RPA protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

RPA/p34 Information for Pathologists

Common Uses By Pathologists:

RPA/p34 is expressed in most cancer cells. It exhibits moderate to strong nuclear staining with additional cytoplasmic positivity in a few cases. Liver cancers showed generally strong immunoreactivity in cholangiocarcinomas. Weaker staining was observed in hepatocellular cancers compared to other malignancies.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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RPA/p34 [9H8] Antibody (cGMP).