Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
|Recommended Dilution (Conc)
||30 min at Room Temperature
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Anti-S-100 Antibody Clone: 4C4.9
Host and Isotype: Mouse IgG2a
Recommended Positive Control Sample: Melanoma
Cellular Localization of Antibody 4C4.9 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Lot specific. Plese contact tech support
Immunogen: BALB/C mice were immunized with purified bovine brain S-100 protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
S-100 Information for Pathologists
Acidic protein, 100% Soluble in ammonium sulfate at neutral pH (derivation of name). S100 protein family is multigenic group of nonubiquitous cytoplasmic EF-hand Ca2+-binding proteins, sharing significant structural similarities at both genomic and protein levels; S100 protein family has 24 known human members each coded by a separate gene; at least 19 on chromosome 1q21 (Hepat Mon 2012;12:e7240). Helps regulate cell contraction, motility, growth, differentiation, cell cycle progression, transcription and secretion. Structurally similar to calmodulin. Interpretation
Common Uses By Pathologists:
Common marker of neural tissue / lesions and melanoma. Differentiate plexiform schwannoma (S100+, Am J Surg Pathol 2005;29:1042) from MPNST (weak / negative / patchy). MelanA and MART1 appear to be superior to S100 for evaluating sentinel lymph nodes for melanoma (Am J Surg Pathol 2001;25:1039). Microscopic (histologic) images Images hosted on PathOut server:.
Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.