FFPE human placenta sections stained with 100 ul anti-S100A4 (clone S100A4/1481) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-S100A4 antibody (Clone S100A4/1481). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Western Blot of HeLa, A549 and A375 Cell Lysate using S1A4 Monoclonal Antibody (S1A4/1481).

Anti-S100A4 Antibody Clone S100A4/1481

$ 199.00$ 459.00
Please Select Product Options Below To View The Catalog Number.

SKU: 6275-MSM1
Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
6275-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
6275-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
6275-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 459.00
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SKU: 6275-MSM1-P0
S100A4 General Information
Alternate Names
S100 calcium-binding protein A4, S100A4, S100A4
Molecular Weight
10-12kDa
Chromosomal Location
1q21
Curated Database and Bioinformatic Data
Gene SymbolS100A4
Entrez Gene ID6275
Ensemble Gene IDENSG00000196154
RefSeq Protein Accession(s)NP_002952, NP_062427
RefSeq mRNA Accession(s)NM_002961, NM_019554
RefSeq Genomic Accession(s)NG_027993, NC_018912, NC_000001
UniProt ID(s)P26447
UniGene ID(s)P26447
HGNC ID(s)10494
Cosmic ID(s)S100A4
KEGG Gene ID(s)hsa:6275
PharmGKB ID(s)PA34906
General Description of S100A4.
S100A4 belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including P53, P63, non-muscle myosin IIA, ?6?4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.

Human and Mouse Anti-S100A4 Antibody Product Attributes

Species: Human and Mouse
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-S100A4 Antibody Clone: S100A4/1481
Clone S100A4/1481 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Mouse S100A4 Positive Control Sample: HeLa, A549 or A375 Cells. Placenta
Cellular Localization of Antibody Cytoplasmic and Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human S100A4 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

S100A4 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-S100A4 antibody staining is expected to be primarily localized to the plasma membrane. There is variability in either the signal strength or the localization of signal in plasma membrane from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in S100A4 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow, macrophages in lung, non-germinal center cells in the lymph node, decidual cells in the placenta, cells in the red pulp in spleen and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow, macrophages in lung, non-germinal center cells in the lymph node, decidual cells in the placenta, cells in the red pulp in spleen and non-germinal center cells in the tonsil. Low, but measureable presence of S100A4 could be seen inlymphoid tissue in appendix, peripheral nerve in mesenchymal tissue and squamous epithelial cells in the tonsil. We were unable to detect S100A4 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of S100A4 expression as measured by anti-S100A4 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - - -
S100A4 Variability + + + ++ ++ + + + ++ ++ ++ ++ ++ + + + ++ + ++ ++

Limitations and Warranty

enQuire Bio's S100A4 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-S100A4 Antibody Clone S100A4/1481

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