Formalin-fixed, paraffin embedded human tonsil sections stained with 100 ul anti-S100A9 (clone S100A9/1011) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.

Formalin-fixed, paraffin-embedded human Tonsil stained with S1A9 Monoclonal Antibody (S1A9/111)

Anti-S100A9 Antibody Clone S100A9/1011

Name: Anti-S100A9 Antibody Clone S100A9/1011
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

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SKU: 6280-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
6280-MSM1-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 6280-MSM1-P1

S100A9 General Information
Alternate Names
S100 calcium-binding protein A9, S100A9, migration inhibitory factor-related protein 14, MRP14, calgranulin B, S100A9
Molecular Weight
14kDa
Chromosomal Location
1q21
Curated Database and Bioinformatic Data
Gene Symbol	S100A9
Entrez Gene ID	6280
Ensemble Gene ID	ENSG00000163220
RefSeq Protein Accession(s)	NP_002956
RefSeq mRNA Accession(s)	NM_002965
RefSeq Genomic Accession(s)	NC_018912, NC_000001
UniProt ID(s)	P06702
UniGene ID(s)	P06702
HGNC ID(s)	10499
Cosmic ID(s)	S100A9
KEGG Gene ID(s)	hsa:6280
PharmGKB ID(s)	PA34911
General Description of S100A9.
Recognizes a 14kDa protein, identified as S100A9 (also known as Calgranulin B or MRP-14); expressed by granulocytes, monocytes, by tissue macrophages.The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm,/or nucleus of a wide range of cells,, involved in the regulation of a number of cellular processes such as cell cycle progression, differentiation. Altered expression of this protein is associated with the disease cystic fibrosis. This MAb reacts with neutrophils, monocytes, macrophages,, has been shown as an important marker for identifying macrophages in tissue sections. Among cells that are now recognized as macrophages are histiocytes, Kupffer cells, osteoclasts, microglial cells, synovial type A cells, interdigitating cells,, Langerhans cells (in normal tissues), epithelioid cells, Langerhans-type, foreign-body-type multinucleated giant cells (in inflamed tissues).

Human Anti-S100A9 Antibody Product Attributes

Species: Human

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed. Not suitable for frozen tissues.) (0.5-1ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-S100A9 Antibody Clone: S100A9/1011

Clone S100A9/1011 Host and Isotype: Mouse IgM kappa

Anti-Human S100A9 Positive Control Sample: Liver or histiocytoma

Cellular Localization of Antibody Cytoplasmic, Extracellular (secreted), Cell Surface

Buffer and Stabilizer: 10mM PBS with 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Recombinant human S100A9 protein

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

S100A9 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-S100A9 antibody staining is expected to be primarily localized to the cell junctions and cytosol.

Observed Antibody Staining Data By Tissue Type:

Variations in S100A9 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. More moderate antibody staining intensity was present in hematopoietic cells in the bone marrow, squamous epithelial cells in the cervix, uterine, esophagus and oral mucosa, epidermal cells in the skin, cells in the red pulp in spleen and squamous epithelial cells in the tonsil and vagina. Low, but measureable presence of S100A9 could be seen in cells in the white pulp in spleen. We were unable to detect S100A9 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of S100A9 expression as measured by anti-S100A9 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	+	-	+	-	+	-	++	-	-	-	-	-	-	++	-	-	-	-
S100A9 Variability	++	+	++	+	++	+	++	++	++	++	+	++	++	++	+	++	+	+	+	++

Limitations and Warranty

enQuire Bio's S100A9 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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