Human Anti-S100B Antibody Product Attributes
Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-S100B Antibody Clone: 4C4.9
Clone 4C4.9 Host and Isotype: Mouse IgG2a kappa
Anti-Human S100B Positive Control Sample: Brain, Melanoma
Cellular Localization of Antibody <4C4.9 Staining: Cytoplasmic and Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Purified bovine brain S100 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
S100B Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-S100B antibody staining is expected to be primarily localized to the nucleus and vesicles.Observed Antibody Staining Data By Tissue Type:
Variations in S100B antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, peripheral nerve/ganglion in colon, glial cells in the hippocampus, neuronal cells in the hippocampus, Langerhans in skin and peripheral nerve in mesenchymal tissue. More moderate antibody staining intensity was present in myoepithelial cells in the breast, glial cells in the caudate nucleus, neuronal cells in the caudate nucleus, cells in the granular layer in cerebellum, cells in the molecular layer in cerebellum, Purkinje cells in the cerebellum, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, neuropil in cerebral cortex, peripheral nerve/ganglion in colon, glial cells in the hippocampus, neuronal cells in the hippocampus, Langerhans in skin and peripheral nerve in mesenchymal tissue. Low, but measureable presence of S100B could be seen in. We were unable to detect S100B in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of S100B expression as measured by anti-S100B antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | ++ | + | - | + | + | ++ | - | - | - | - | ++ | + | ++ | + | - | - | + | - | + | + |
S100B Variability | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | + | + | +++ | ++ | + | ++ | + | + | ++ | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's S100B Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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