Immunohistochemical staining of Smooth Muscle Myosin  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Smooth Muscle Myosin [SMMS-1] Antibody (cGMP).

$ 135.00$ 495.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD188
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD188-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD188-0.5ml Size: 0.5 ml, Format: Concentrate $ 335.00
QIVD188-1ml Size: 1 ml, Format: Concentrate $ 495.00
QIVD188-6ml Size: 6 ml, Format: Predilute $ 285.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

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SKU: QIVD188-0.1ml
Smooth Muscle Myosin General Information
Alternate Names
Molecular Weight
210.7 kDa
Chromosomal Location
q21.1 [chr: 3] [chr_start: 123610049] [chr_end: 123884331] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolMYLK
Entrez Gene ID4638
RefSeq Protein Accession(s)NP_444254; XP_016861961; NP_444253; NP_444259; XP_016861960; NP_001308238; NP_444260; NP_444255; XP_011511162; XP_016861962; XP_016861958; NP_444256
RefSeq mRNA Accession(s)NM_001321309; NM_053028; XM_017006472; XM_024453534; XM_024453537; NM_053026; NM_053032; XM_024453536; NM_053027; NM_005965; XM_017006469; XM_011512860; XM_011512861; XM_017006473; XM_024453532; XM_024453535; NM_053025; NM_053031; NM_053029; XM_017006470; XM_024453533; NM_053030; XM_017006471
RefSeq Genomic Accession(s)NC_000003; NG_029111;
UniProt ID(s)Q15746
PharmGKB ID(s)PA31388
KEGG Gene ID(s)hsa:4638
Associated Diseases (KEGG IDs)Aortic aneurysm, familial thoracic 7 (AAT7) [MIM:613780]: A disease characterized by permanent dilation of the thoracic aorta usually due to degenerative changes in the aortic wall. It is primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. {ECO:0000269|PubMed:21055718}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of Smooth Muscle Myosin .
Monoclonal anti-smooth muscle myosin reacts with myosin heavy chain polypeptides of 200 and 204 kDa. It does not react with skeletal, cardiac, or non-muscle myosin. In immunohistochemistry it stains vascular and visceral smooth muscle cells and cells that have smooth muscle-like characteristics.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:100-1:500
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Smooth Muscle Myosin Antibody Clone: SMMS-1
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Colon, appendix
Cellular Localization of Antibody SMMS-1 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Human uterus smooth muscle extract
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Smooth Muscle Myosin Information for Pathologists

Summary:

Smooth Muscle Myosin (heavy chain, also known as SMMHC) is encoded by the MYH11 gene. Fusion between core binding factor beta (CBF-beta) with SMMHC (CBFbeta-SMMHC; Inv(16)(p13q22)) is present in nearly all acute myeloid leukemia M4 eosinophilia subtype (AML M4Eo). Smooth Muscle Myosin is useful for differentiating between benign and malignant lesions.

Common Uses By Pathologists:

SMM is present in acute myeloid leukemia of M4 eosinophilia subtype. SMM IHC staining can be used to evaluate restenosis in peripheral artery disease. Its expression is decreased in diverticular disease.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Smooth Muscle Myosin [SMMS-1] Antibody (cGMP).