Human, Rat, and Broad Species Reactivity Anti-Smooth Muscle Myosin Heavy Chain Antibody Product Attributes
Species: Human, Rat, and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-Smooth Muscle Myosin Heavy Chain Antibody Clone: MYH11/923 + SMMS-1
Clone MYH11/923 + SMMS-1 Host and Isotype: Mouse IgG
Anti-Human, Rat, and Broad Species Reactivity Smooth Muscle Myosin Heavy Chain Positive Control Sample: Uterus or normal breast
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human MYH11 protein (MYH11/923); Human uterus extract (SMMS-1)
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Smooth Muscle Myosin Heavy Chain Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-MYH11 antibody staining is expected to be primarily localized to the cytosol and plasma membrane.
Observed Antibody Staining Data By Tissue Type:
Variations in Smooth Muscle Myosin Heavy Chain antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. Low, but measureable presence of Smooth Muscle Myosin Heavy Chain could be seen in. We were unable to detect Smooth Muscle Myosin Heavy Chain in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's Smooth Muscle Myosin Heavy Chain Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.