Human Anti-SOX10 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-SOX10 Antibody Clone: SPM607
Clone SPM607 Host and Isotype: Mouse IgG2b kappa
Anti-Human SOX10 Positive Control Sample: HepG2 cells. Melanomas, breast carcinomas, gliomas.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (aa 115-269) of human SOX10 protein with hexa-histidine tag
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
SOX10 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-SOX10 antibody staining is expected to be primarily localized to the nucleoplasm. There is variability in either the signal strength or the localization of signal in nucleoplasm from cell to cell.Observed Antibody Staining Data By Tissue Type:
Variations in SOX10 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glial cells in the caudate nucleus and hippocampus, glandular cells in the salivary gland and melanocytes in skin. More moderate antibody staining intensity was present in glial cells in the caudate nucleus and hippocampus, glandular cells in the salivary gland and melanocytes in skin. Low, but measureable presence of SOX10 could be seen inglandular cells in the breast, cells in the granular layer in cerebellum, keratinocytes in skin, Langerhans in skin, epidermal cells in the skin, peripheral nerve in mesenchymal tissue and chondrocytes in mesenchymal tissue. We were unable to detect SOX10 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of SOX10 expression as measured by anti-SOX10 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Signal Intensity | - | - | - | - | - | + | - | - | - | - | + | - | - | - | - | - | - | - | - | - |
SOX10 Variability | + | + | + | + | + | ++ | + | + | + | + | ++ | + | + | + | + | + | + | + | + | + |
Limitations and Warranty
enQuire Bio's SOX10 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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