Formalin-fixed, paraffin-embedded human Melanoma stained with SOX1 Monoclonal Antibody (SPM67).
Formalin-fixed, paraffin-embedded human Melanoma stained with SOX1 Monoclonal Antibody (SPM67).

Anti-SOX10 Antibody Clone SPM607

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 6663-MSM3X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
6663-MSM3X-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 6663-MSM3X-P1
SOX10 General Information
Alternate Names
Transcription factor SOX-10, SOX10
Molecular Weight
49-58kDa
Chromosomal Location
22q13.1
Curated Database and Bioinformatic Data
Gene SymbolSOX10
Entrez Gene ID6663
Ensemble Gene IDENSG00000100146
RefSeq Protein Accession(s)NP_008872
RefSeq mRNA Accession(s)NM_006941
RefSeq Genomic Accession(s)NG_007948, NC_000022, NC_018933
UniProt ID(s)P56693, A0A024R1N6
UniGene ID(s)P56693, A0A024R1N6
HGNC ID(s)11190
Cosmic ID(s)SOX10
KEGG Gene ID(s)hsa:6663
PharmGKB ID(s)PA36027
General Description of SOX10.
Recognizes a protein of ~55kDa, identified as SOX10. This MAb is highly specific, does not cross-react with other members of the SOX-family. SOX genes comprise a family of genes that are related to the mammalian sex-determining gene SRY. These genes similarly contain sequences that encode for the HMG-box domain, which is responsible for the sequence-specific DNA-binding activity. SOX-10 is a sensitive marker of melanoma, including conventional, spindled,, desmoplastic subtypes. It is expressed by metastatic melanomas, nodal capsular nevus in sentinel lymph nodes, but not by other lymph node components such as dendritic cells, which usually express S100 protein. Commonly used melanoma markers, such as anti-HMB-45, anti-Melan-A, are poorly expressed in desmoplastic melanomas while SOX-10 is moderately to strongly expressed in desmoplastic melanomas. SOX-10 is considered as a very reliable marker for recognizing residual desmoplastic melanomas. In normal tissues, it is expressed in Schwann cells, melanocytes,, myoepithelial cells of salivary, bronchial, mammary glands. SOX-10 expression is also observed in mast cells.

Human Anti-SOX10 Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-SOX10 Antibody Clone: SPM607
Clone SPM607 Host and Isotype: Mouse IgG2b kappa
Anti-Human SOX10 Positive Control Sample: HepG2 cells. Melanomas, breast carcinomas, gliomas.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (aa 115-269) of human SOX10 protein with hexa-histidine tag
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

SOX10 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-SOX10 antibody staining is expected to be primarily localized to the nucleoplasm. There is variability in either the signal strength or the localization of signal in nucleoplasm from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in SOX10 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glial cells in the caudate nucleus and hippocampus, glandular cells in the salivary gland and melanocytes in skin. More moderate antibody staining intensity was present in glial cells in the caudate nucleus and hippocampus, glandular cells in the salivary gland and melanocytes in skin. Low, but measureable presence of SOX10 could be seen inglandular cells in the breast, cells in the granular layer in cerebellum, keratinocytes in skin, Langerhans in skin, epidermal cells in the skin, peripheral nerve in mesenchymal tissue and chondrocytes in mesenchymal tissue. We were unable to detect SOX10 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of SOX10 expression as measured by anti-SOX10 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - + - - - - + - - - - - - - - -
SOX10 Variability + + + + + ++ + + + + ++ + + + + + + + + +

Limitations and Warranty

enQuire Bio's SOX10 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-SOX10 Antibody Clone SPM607

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