Formalin-fixed, paraffin embedded mouse brain sections stained with 100 ul anti-SOX2 (clone SOX2/1791) at 1:300. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin embedded mouse intestine sections stained with 100 ul anti-SOX2 (clone SOX2/1791) at 1:100. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Formalin-fixed, paraffin embedded mouse stomach sections stained with 100 ul anti-SOX2 (clone SOX2/1791) at 1:50. HIER epitope retrieval prior to staining was performed in 10mM Citrate, pH 6.0.Data from SDS-PAGE analysis of Anti-SOX2 antibody (Clone SOX2/1791). Reducing lane (R) shows heavy and light chain fragments. NR lane shows intact antibody with expected MW of approximately 150 kDa. The data are consistent with a high purity, intact mAb.Formalin-fixed, paraffin-embedded Human Cervical Carcinoma stained with SOX2 Monoclonal Antibody (SOX2/1791).

Anti-SOX2 Antibody Clone SOX2/1791

$ 228.00$ 518.00
Please Select Product Options Below To View The Catalog Number.

SKU: 6657-MSM1
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
6657-MSM1-P0 Size: 20 ug, Tag: Unconjugated $ 228.00
6657-MSM1-P1 Size: 100 ug, Tag: Unconjugated $ 488.00
6657-MSM1-P2 Size: 200 ug, Tag: Unconjugated $ 518.00
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SKU: 6657-MSM1-P0
SOX2 General Information
Alternate Names
SRY, sex determining region Y-box 2, SOX2, Transcription Factor SOX-2
Molecular Weight
34kDa
Chromosomal Location
3q26.33
Curated Database and Bioinformatic Data
Gene SymbolSOX2
Entrez Gene ID6657
Ensemble Gene IDENSG00000181449
RefSeq Protein Accession(s)NP_003097
RefSeq mRNA Accession(s)NM_003106,
RefSeq Genomic Accession(s)NC_018914, NG_009080, NC_000003
UniProt ID(s)A0A0U3FYV6, P48431
UniGene ID(s)A0A0U3FYV6, P48431
HGNC ID(s)11195
Cosmic ID(s)SOX2
KEGG Gene ID(s)hsa:6657
PharmGKB ID(s)PA36032
General Description of SOX2.
SOX2 is a member of the SRY-related HMG-box (SOX) family of transcription factors involved in the regulation of embryonic development and in the determination of cell fate. At present, 30 Sox genes have been identified. SOX2 is required for stem cell maintenance in the central nervous system, and it also regulates gene expression in the stomach. SOX2 is necessary for regulating multiple transcription factors that affect Oct 3/4 expression. An essential function of SOX2 is to stabilize embryonic stem cells in a pluripotent state by maintaining the requisite level of Oct 3/4 expression.

Human Anti-SOX2 Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-SOX2 Antibody Clone: SOX2/1791
Clone SOX2/1791 Host and Isotype: Mouse IgG2b kappa
Anti-Human SOX2 Positive Control Sample: MCF-7 cells or Cervical carcinoma
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human SOX2 protein fragment (aa176-305)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

SOX2 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-SOX2 antibody staining is expected to be primarily localized to the nucleoplasm.

Observed Antibody Staining Data By Tissue Type:

Variations in SOX2 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of SOX2 could be seen inrespiratory epithelial cells in the bronchus, glial cells in the cerebral cortex, neuronal cells in the cerebral cortex, squamous epithelial cells in the cervix, uterine and esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, fibroblasts in mesenchymal tissue, germinal center cells in the tonsil and squamous epithelial cells in the vagina. We were unable to detect SOX2 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of SOX2 expression as measured by anti-SOX2 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - + + + - + + - - - - - - - - - - - - -
SOX2 Variability ++ ++ ++ ++ + ++ ++ ++ ++ + + + + + + + + ++ ++ ++

Limitations and Warranty

enQuire Bio's SOX2 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-SOX2 Antibody Clone SOX2/1791

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