Analysis of Mass Spec data (dashed-line) of fractions stained with SUMO-1 MS-QAVA™ monoclonal antibody [Clone: SM1/495] (solid-line), reveals that less than 9.8% of signal is attributable to non-specific binding of anti-SUMO-1  [Clone SM1/495] to targets other than SUMO1 protein. Even frequently cited antibodies have much greater non-specific interactions, averaging over 30%. Data in image is from analysis in A431, RT4 and MCF7 cells.

Anti-SUMO-1 Antibody Clone SM1/495

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7341-MSM1
Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: MS-QAVA™ Validated

Product NumberDescriptionPrice
7341-MSM1-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 7341-MSM1-P1
SUMO-1 General Information
Alternate Names
Small ubiquitin-related modifier 1, SUMO1, SUMO-1
Molecular Weight
11.5kDa (Monomer); 90kDa (Heteromer)
Chromosomal Location
2q33.1
Curated Database and Bioinformatic Data
Gene SymbolSUMO1
Entrez Gene ID7341
Ensemble Gene IDENSG00000116030
RefSeq Protein Accession(s)NP_003343, NP_001005781, NP_001005782
RefSeq mRNA Accession(s)NM_003352, NM_001005782 NM_001005781
RefSeq Genomic Accession(s)NG_011679, NC_000002, NC_018913
UniProt ID(s)P55856, A0A024R3Z2, Q93068, P63165
UniGene ID(s)P55856, A0A024R3Z2, Q93068, P63165
HGNC ID(s)12502
Cosmic ID(s)SUMO1
KEGG Gene ID(s)hsa:7341
PharmGKB ID(s)PA37149
General Description of SUMO-1.
This MAb is specific to SUMO-1, shows no cross-reaction with either SUMO-2 or SUMO-3. The small ubiquitin-related modifier (SUMO) proteins, which include SUMO-1, SUMO-2, SUMO-3, belong to the ubiquitin-like protein family. Like ubiquitin, the SUMO proteins are synthesized as precursor proteins that undergo processing before conjugation to target proteins. Also, both utilize the E1, E2,, E3 cascade enzymes for conjugation. However, SUMO, ubiquitin differ with respect to targeting. Ubiquitination predominantly targets proteins for degradation, whereas sumoylation targets proteins to a variety of cellular processing, including nuclear transport, transcriptional regulation, apoptosis, protein stability. The unconjugated SUMO-1 protein localizes to the nuclear membrane.

Human and Rat Anti-SUMO-1 Antibody Product Attributes

Species: Human and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry (IHC). Additionally, this antibody has been validated by MS-QAVA™. Specificity has been quantitatively determined via Mass Spec.
Clonality: Monoclonal
Anti-SUMO-1 Antibody Clone: SM1/495
Clone SM1/495 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat SUMO-1 Positive Control Sample: Breast carcinoma
Cellular Localization of Antibody Predominantly nuclear with some cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human SUMO1 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

SUMO-1 Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Variations in SUMO-1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the adrenal gland, hematopoietic cells in the bone marrow, adipocytes in breast, respiratory epithelial cells in the bronchus, glandular cells in the duodenum, endometrium, epididymis, fallopian tube and gallbladder, macrophages in lung, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the rectum, myocytes in skeletal muscle, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, glandular cells in the small intestine, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, cells in the seminiferous ducts in testis, germinal center cells in the tonsil, non-germinal center cells in the tonsil and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in glandular cells in the adrenal gland, hematopoietic cells in the bone marrow, adipocytes in breast, respiratory epithelial cells in the bronchus, glandular cells in the duodenum, endometrium, epididymis, fallopian tube and gallbladder, macrophages in lung, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the rectum, myocytes in skeletal muscle, fibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, glandular cells in the small intestine, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, cells in the seminiferous ducts in testis, germinal center cells in the tonsil, non-germinal center cells in the tonsil and urothelial cells in the urinary bladder. Low, but measureable presence of SUMO-1 could be seen inglandular cells in the appendix, lymphoid tissue in appendix, glial cells in the caudate nucleus and cerebral cortex, bile duct cells in the liver and Leydig cells in the testis. We were unable to detect SUMO-1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Type:

Tissues from cancer patients, for instance, have their own distinct pattern of SUMO-1 expression as measured by anti-SUMO-1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + ++ ++ + ++ ++ ++ + ++ ++ +++ ++ ++ + ++ ++ +++ ++ ++
SUMO1 Variability ++ ++ ++ ++ +++ ++ ++ +++ ++ ++ +++ ++ ++ ++ +++ ++ ++ ++ ++ +++

Limitations and Warranty

This product is for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-SUMO-1 Antibody Clone SM1/495

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