Human and Rat (-) Anti-Tenascin C Antibody Product Attributes
Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Tenascin C Antibody Clone: T2H5
Clone T2H5 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat (-) Tenascin C Positive Control Sample: Extracellular matrix in tonsil, blood vessels. Stroma of many tumors such as breast, squamous cell,, lung carcinomas. Staining of normal fibroblasts serves as internal positive control
Cellular Localization of Antibody Connective tissue matrix
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human breast carcinoma
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
Tenascin C Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:
Variations in Tenascin C antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Tenascin C could be seen inhematopoietic cells in the bone marrow, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, macrophages in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, ovarian stroma cells in the ovary, glandular cells in the prostate, rectum, salivary gland and seminal vesicle, myocytes in skeletal muscle, keratinocytes in skin, epidermal cells in the skin, cells in the red pulp in spleen, glandular cells in the stomach, germinal center cells in the tonsil and non-germinal center cells in the tonsil. We were unable to detect Tenascin C in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of Tenascin C expression as measured by anti-Tenascin C antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | ++ | - | ++ | - | - | ++ | ++ | - | + | - | ++ | ++ | - | - | - | ++ | - | - | ++ | ++ |
| TNC Variability | ++ | ++ | ++ | ++ | ++ | + | + | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ | ++ |
Limitations and Warranty
enQuire Bio's Tenascin C Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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