Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Tenascin C Monoclonal Antibody (T2H5) at 4ug/ml. Antigen retrieval in 1mM Tris with 1mM EDTA, pH 9.; ABC detection system with DAB Chromogen. Note staining of connective tissue.
Formalin-fixed, paraffin-embedded human Lung Carcinoma stained with Tenascin C Monoclonal Antibody (T2H5) at 4ug/ml. Antigen retrieval in 1mM Tris with 1mM EDTA, pH 9.; ABC detection system with DAB Chromogen. Note staining of connective tissue.

Anti-Tenascin C Antibody Clone T2H5

$ 298.00
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SKU: 3371-MSM1
Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

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Datasheets and Documentation
Product Datasheet
SKU: 3371-MSM1-P1
Tenascin C General Information
Alternate Names
TNC, Tenascin C, TN-C
Molecular Weight
210kDa, 300kDa
Chromosomal Location
9q33
Curated Database and Bioinformatic Data
Gene SymbolTNC
Entrez Gene ID3371
Ensemble Gene IDENSG00000041982
RefSeq Protein Accession(s)XP_016870167, XP_005252030, XP_006717164, XP_006717159, XP_005252029, XP_006717161, XP_005252031, XP_005252032, XP_011516928, XP_011516930, XP_016870168, XP_016870170, XP_006717160, XP_011516927, XP_011516931, XP_016870169, NP_002151
RefSeq mRNA Accession(s)XM_017014679, XM_017014681, XM_011518629, XM_011518626, XM_005251972, XM_006717097, XM_005251975, XM_011518625, XM_006717096, XM_011518628, XM_017014678, XM_017014680, NM_002160, XM_005251973, XM_006717098, XM_006717101
RefSeq Genomic Accession(s)NC_018920, NC_000009, NG_029637
UniProt ID(s)B4E1W8, P24821, Q4LE33, J3QSU6
UniGene ID(s)B4E1W8, P24821, Q4LE33, J3QSU6
HGNC ID(s)5318
Cosmic ID(s)TNC
KEGG Gene ID(s)hsa:3371
PharmGKB ID(s)PA35103
General Description of Tenascin C.
In Western Blot, it reacts with two bands of ~MW of 210kDa, 300kDa, identified as two isoforms of Tenascin C. Specificity of this MAb is validated by sequential immunoprecipitation with a PAb against Tenascin C. Tenascin C is a multifunctional, disulfide-linkedhexameric extracellular matrix glycoprotein expressed in association with mesenchymal epithelial interactions during development, in the neo-vasculature, stroma of undifferentiated tumors. In adults, it is restricted to certain epithelial-stromal interfaces, increases markedly in hyper-proliferative diseases, in stroma of many neoplasms, including gliomas, breast, squamous, lung carcinomas.

Human and Rat (-) Anti-Tenascin C Antibody Product Attributes

Species: Human and Rat (-)
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (2-4ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Tenascin C Antibody Clone: T2H5
Clone T2H5 Host and Isotype: Mouse IgG1 kappa
Anti-Human and Rat (-) Tenascin C Positive Control Sample: Extracellular matrix in tonsil, blood vessels. Stroma of many tumors such as breast, squamous cell,, lung carcinomas. Staining of normal fibroblasts serves as internal positive control
Cellular Localization of Antibody Connective tissue matrix
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human breast carcinoma
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Tenascin C Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Tenascin C antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Tenascin C could be seen inhematopoietic cells in the bone marrow, respiratory epithelial cells in the bronchus, squamous epithelial cells in the cervix, uterine, macrophages in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, ovarian stroma cells in the ovary, glandular cells in the prostate, rectum, salivary gland and seminal vesicle, myocytes in skeletal muscle, keratinocytes in skin, epidermal cells in the skin, cells in the red pulp in spleen, glandular cells in the stomach, germinal center cells in the tonsil and non-germinal center cells in the tonsil. We were unable to detect Tenascin C in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Tenascin C expression as measured by anti-Tenascin C antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ - ++ - - ++ ++ - + - ++ ++ - - - ++ - - ++ ++
TNC Variability ++ ++ ++ ++ ++ + + ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's Tenascin C Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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