Immunohistochemical staining of Terminal Deoxynucleotidyl Transferase  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Terminal Deoxynucleotidyl Transferase [SEN28] Antibody (cGMP).

$ 135.00$ 865.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD192
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG2a, kappa

Product NumberDescriptionPrice
QIVD192-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD192-0.5ml Size: 0.5 ml, Format: Concentrate $ 515.00
QIVD192-1ml Size: 1 ml, Format: Concentrate $ 865.00
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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SKU: QIVD192-0.1ml
Terminal Deoxynucleotidyl Transferase General Information
Alternate Names
Molecular Weight
58.5 kDa
Chromosomal Location
q24.1 [chr: 10] [chr_start: 96304409] [chr_end: 96338564] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolDNTT
Entrez Gene ID1791
RefSeq Protein Accession(s)NP_004079; NP_001017520
RefSeq mRNA Accession(s)NM_001017520; NM_004088
RefSeq Genomic Accession(s)NC_000010
UniProt ID(s)P04053
PharmGKB ID(s)PA27449
KEGG Gene ID(s)hsa:1791
General Description of Terminal Deoxynucleotidyl Transferase .
This antibody stains a 60kDa peptide in human lymphoblasts. Tdt is present in the cells during immunoglobulin gene rearrangement and plays an important role in lymphocyte maturation. Tdt marks both T and B immature lymphocytes normally found in thymus and bone marrow.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:10-1:50
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Terminal Deoxynucleotidyl Transferase Antibody Clone: SEN28
Host and Isotype: Mouse IgG2a, kappa
Recommended Positive Control Sample: Thymus
Cellular Localization of Antibody SEN28 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with Tdt.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Terminal Deoxynucleotidyl Transferase Information for Pathologists

Summary:

Terminal deoxynucleotidyl transferase; also called terminal transferase. Nuclear DNA polymerase in thymic and small number of bone marrow cortical lymphocytes. Catalyzes addition of deoxynucleotides to 3' OH ends of oligonucleotides or polydeoxynucleotides. Performs DNA synthesis using only single-stranded DNA as the nucleic acid substrate (Biochim Biophys Acta 2010;1804:1151). Used during immunoglobulin and T cell receptor rearrangement, adds diversity to antibody recognition sites.

Common Uses By Pathologists:

Interpretation: nuclear stain. Marker of B or T cell acute lymphoblastic leukemia/lymphoma (only rarely negative, Arch Pathol Lab Med 2000;124:92). Microscopic (histologic) images Image hosted on PathOut server:.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Terminal Deoxynucleotidyl Transferase [SEN28] Antibody (cGMP).