Formalin-fixed, paraffin-embedded human Placenta stained with Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (SPM322).
Formalin-fixed, paraffin-embedded human Placenta stained with Thymidine Phosphorylase / PD-ECGF Monoclonal Antibody (SPM322).

Anti-Thymidine Phosphorylase / PD-ECGF Antibody Clone SPM322

$ 118.00$ 418.00
Please Select Product Options Below To View The Catalog Number.

SKU: 1890-MSM1X
Species: Human, Mouse, and Rat
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
1890-MSM1X-P0 Size: 20 ug, Tag: Unconjugated $ 118.00
1890-MSM1X-P1 Size: 100 ug, Tag: Unconjugated $ 298.00
1890-MSM1X-P2 Size: 200 ug, Tag: Unconjugated $ 418.00
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SKU: 1890-MSM1X-P0
Thymidine Phosphorylase / PD-ECGF General Information
Alternate Names
Thymidine Phosphorylase, TYMP gene, ECGF1, Platelet-derived endothelial cell growth factor
Molecular Weight
55kDa
Chromosomal Location
22q13.33
Curated Database and Bioinformatic Data
Gene SymbolTYMP
Entrez Gene ID1890
Ensemble Gene IDENSG00000025708
RefSeq Protein Accession(s)NP_001944, NP_001107228, NP_001244918, NP_001244917, NP_001107227
RefSeq mRNA Accession(s)NM_001257988, NM_001113756, NM_001257989 NM_001113755, NM_001953
RefSeq Genomic Accession(s)NG_011860, NC_018933, NC_000022, NG_016235
UniProt ID(s)P19971, E5KRG5, B2RBL3
UniGene ID(s)P19971, E5KRG5, B2RBL3
HGNC ID(s)3148
Cosmic ID(s)TYMP
KEGG Gene ID(s)hsa:1890
PharmGKB ID(s)PA162407502
General Description of Thymidine Phosphorylase / PD-ECGF.
Recognizes a protein (amino acid 482) of 55kDa (in vivo 110kDa homodimer), identified as platelet-derived endothelial growth factor (PD-ECGF), same as thymidine phosphorylase (TP) or gliostatin. In the presence of inorganic orthophosphate, it catalyzes the reversible phospholytic cleavage of thymidine and deoxyuridine to their corresponding bases and 2-deoxyribose-1-phosphate. It is both chemotactic and mitogenic for endothelial cells and a non-heparin binding angiogenic factor present in platelets. Its enzymatic activity is crucial for angiogenic activity (metabolite is angiogenic). Higher levels of serum TP/PD-ECGF are observed in cancer patients. It is also involved in transformation of fluoropyrimidines, cytotoxic agents used in the treatment of a variety of malignancies, into active cytotoxic metabolites (e.g. 5 -deoxy-5-fluorouridine to 5-FU). High intra-cellular levels of TP/PD-ECGF are associated with increased chemosensitivity to such antimetabolites.

Human, Mouse, and Rat Anti-Thymidine Phosphorylase / PD-ECGF Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Thymidine Phosphorylase / PD-ECGF Antibody Clone: SPM322
Clone SPM322 Host and Isotype: Mouse IgG1
Anti-Human, Mouse, and Rat Thymidine Phosphorylase / PD-ECGF Positive Control Sample: HUVEC cells. Breast, Bladder, Lung or Kaposi tumors
Cellular Localization of Antibody Cytoplasmic and Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human Thymidine Phosphorylase (TP / PD-ECGF) protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Thymidine Phosphorylase / PD-ECGF Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TYMP antibody staining is expected to be primarily localized to the golgi apparatus and nuclear bodies.

Observed Antibody Staining Data By Tissue Type:

Variations in Thymidine Phosphorylase / PD-ECGF antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. Low, but measureable presence of Thymidine Phosphorylase / PD-ECGF could be seen inendothelial cells in the cerebral cortex, glial cells in the cerebral cortex, cells in the endometrial stroma in endometrium, glandular cells in the endometrium, cells in the tubules in kidney, glandular cells in the prostate and seminal vesicle, keratinocytes in skin, melanocytes in skin, non-germinal center cells in the tonsil and urothelial cells in the urinary bladder. We were unable to detect Thymidine Phosphorylase / PD-ECGF in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Thymidine Phosphorylase / PD-ECGF expression as measured by anti-Thymidine Phosphorylase / PD-ECGF antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity + - ++ + - + ++ + - + + + - - - - - + + +
TYMP Variability +++ + ++ +++ ++ ++ ++ ++ ++ ++ +++ ++ ++ ++ ++ ++ ++ ++ ++ ++

Limitations and Warranty

enQuire Bio's Thymidine Phosphorylase / PD-ECGF is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Thymidine Phosphorylase / PD-ECGF Antibody Clone SPM322

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