Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Thyroglobulin Monoclonal Antibody (SPM221).
Formalin-fixed, paraffin-embedded human Thyroid Carcinoma stained with Thyroglobulin Monoclonal Antibody (SPM221).

Anti-Thyroglobulin Antibody Clone SPM221

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7038-MSM1X
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7038-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: 7038-MSM1X-P1
Thyroglobulin General Information
Alternate Names
Thyroglobulin, Tg, Thyroid Hormone Precursor
Molecular Weight
660kDa (Dimeric Form)
Chromosomal Location
8q24.22
Curated Database and Bioinformatic Data
Gene SymbolTG
Entrez Gene ID7038
Ensemble Gene IDENSG00000042832
RefSeq Protein Accession(s)XP_016869288, XP_006716685, XP_016869285, XP_005251097, XP_005251099, XP_016869283, XP_016869282, XP_016869289, XP_005251095, XP_016869284, XP_016869286, XP_016869287, NP_003226
RefSeq mRNA Accession(s)XM_017013794, XM_017013800, XM_006716622 NM_003235, XM_017013795, XM_017013798, XM_005251040, XM_017013799, XM_017013793, XM_005251038, XM_017013796, XM_017013797, XM_005251042
RefSeq Genomic Accession(s)NG_015832, NC_018919, NC_000008
UniProt ID(s)P01266
UniGene ID(s)P01266
HGNC ID(s)11764
Cosmic ID(s)TG
KEGG Gene ID(s)hsa:7038
PharmGKB ID(s)PA36479
General Description of Thyroglobulin.
Thyroglobulin is a 660kDa dimeric pre-protein with mutiple glycosylation sites. It is produced by, processed within the thyroid gland to produce the hormone thyroxine, triiodothyronine. Prior to forming dimers, thyroglobulin monomers undergo conformational maturation in the endoplasmic reticulation. The vast majority of follicular carcinomas of the thyroid will give positive immunoreactivity for anti-thyroglobulin even though sometimes only focally. Poorly differentiated carcinomas of the thyroid are frequently anti-thyroglobulin negative. Adenocarcinomas of other-than-thyroid origin do not react with this antibody. This antibody is useful in identification of thyroid carcinoma of the papillary, follicular types. Presence of thyroglobulin in metastatic lesions establishes the thyroid origin of tumor. Anti-thyroglobulin, combined with anti-calcitonin, can identify medullary carcinomas of the thyroid. Furthermore, anti-thyroglobulin, combined with anti-TTF1, can be a reliable marker to differentiate between primary thyroid, lung neoplasms.

Human, Mouse, and Rat Anti-Thyroglobulin Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.1-0.2ug of antibody/ml, 30 min at RT)
Clonality: Monoclonal
Anti-Thyroglobulin Antibody Clone: SPM221
Clone SPM221 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, and Rat Thyroglobulin Positive Control Sample: Thyroid
Cellular Localization of Antibody Cytoplasmic, secreted
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human thyroid follicular cells
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Thyroglobulin Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in Thyroglobulin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the thyroid gland. More moderate antibody staining intensity was present in glandular cells in the thyroid gland. Low, but measureable presence of Thyroglobulin could be seen in. We were unable to detect Thyroglobulin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Thyroglobulin expression as measured by anti-Thyroglobulin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - - - - - - - - - +++ -
TG Variability + + + + + + + + + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's Thyroglobulin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Thyroglobulin Antibody Clone SPM221

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