Immunohistochemical staining of Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker [8G7G3/1] Antibody (cGMP).

$ 135.00$ 715.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD198
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1

Product NumberDescriptionPrice
QIVD198-0.1ml Size: 0.1 ml, Format: Concentrate $ 135.00
QIVD198-0.5ml Size: 0.5 ml, Format: Concentrate $ 425.00
QIVD198-1ml Size: 1 ml, Format: Concentrate $ 715.00
Shipping Information
In Stock
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

Expiration Date:

Concentration (Write Lyophilized if Lyophilized):

Reconsitution Instructions (Leave Blank if Liquid):

Manufacture Date:

Purity:

Bioactivity (test results eg. IU/ml):


SKU: QIVD198-0.1ml
Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker General Information
Alternate Names
Molecular Weight
38.6 kDa
Chromosomal Location
q13.3 [chr: 14] [chr_start: 36516392] [chr_end: 36521149] [strand: -1]
Curated Database and Bioinformatic Data
Gene SymbolNKX2-1
Entrez Gene ID7080
RefSeq Protein Accession(s)NP_003308; NP_001073136
RefSeq mRNA Accession(s)NM_003317; NM_001079668
RefSeq Genomic Accession(s)NG_013365; NC_000014
UniProt ID(s)P43699
PharmGKB ID(s)PA36531
KEGG Gene ID(s)hsa:7080
Associated Diseases (KEGG IDs)Chorea, hereditary benign (BHC) [MIM:118700]: A rare autosomal dominant movement disorder, defined by early onset in childhood, a stable or non-progressive course of chorea, and no mental deterioration. Chorea is characterized by involuntary, forcible, rapid, jerky movements that may be subtle or become confluent, markedly altering normal patterns of movement. {ECO:0000269|PubMed:11971878, ECO:0000269|PubMed:15955952, ECO:0000269|PubMed:24453141, ECO:0000269|PubMed:26723978}. The disease is caused by mutations affecting the gene represented in this entry.; Choreoathetosis and congenital hypothyroidism with or without pulmonary dysfunction (CAHTP) [MIM:610978]: An autosomal dominant disorder that manifests in infancy with neurological disturbances, hypothyroidism, and respiratory problems. It is characterized by movement abnormalities beginning with muscular hypotonia followed by the development of chorea, athetosis, dystonia, ataxia, and dysarthria. {ECO:0000269|PubMed:11854318, ECO:0000269|PubMed:11854319, ECO:0000269|PubMed:15289765, ECO:0000269|PubMed:15955952, ECO:0000269|PubMed:24714694}. The disease is caused by mutations affecting the gene represented in this entry.; Thyroid cancer, non-medullary, 1 (NMTC1) [MIM:188550]: A form of non-medullary thyroid cancer (NMTC), a cancer characterized by tumors originating from the thyroid follicular cells. NMTCs represent approximately 95% of all cases of thyroid cancer and are classified into papillary, follicular, Hurthle cell, and anaplastic neoplasms. {ECO:0000269|PubMed:19176457}. Disease susceptibility is associated with variations affecting the gene represented in this entry.
General Description of Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker .
This antibody is specific to a 40 kDa protein, which is identified as thyroid transcription factor (TTF-1). TTF-1 is expressed in epithelial cells of the thyroid gland and lung. TTF-1 stains primary lung adenocarcinoma and small cell carcinoma but does not stain colon and breast carcinoma.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:75
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker Antibody Clone: 8G7G3/1
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Lung
Cellular Localization of Antibody 8G7G3/1 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with a recombinant rat protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker Information for Pathologists

Summary:

Thyroid transcription factor, also called thyroid specific enhancer binding protein. 38 kDa nuclear protein that regulates transcription activity of thyroid (thyroglobulin, thyroperoxidase, sodium-iodide transport protein, calcitonin and MHC class I), lung (surfactant proteins A, B and C, Clara cell secretory protein) and diencephalon specific genes. Mutations cause pulmonary hypoplasia and neonatal death. Specificity depends on antibody clone used. Interpretation: nuclear stain.

Common Uses By Pathologists:

Distinguish primary (TTF1+) vs. metastatic (usually TTF1-) lung carcinoma. Distinguish pulmonary adenocarcinoma (TTF1+) from squamous cell carcinoma (usually TTF1-). Pleural lung carcinoma (TTF1+) vs. mesothelioma (TTF1-). Pulmonary small cell carcinoma (TTF1+) vs. Merkel cell carcinoma (TTF1-). Microscopic (histologic) images

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

There are no reviews yet.

Be the first to review “Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker [8G7G3/1] Antibody (cGMP).”

Your email address will not be published. Required fields are marked *

Share a Protocol (View Reviewed Protocols Below)

Protocol Title

Materials

Methods

Notes


  protocol submission gif
enQuire Bio, LLC   8420 S Continental Divide Rd, #202   Littleton, CO 80127
Phone: 1-855-344-1400 | enquire@enquirebio.com | Fax: 1-720-897-3730
Customer Service Available M-F 8AM-6PM MST or 24/7 Via Email

Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker [8G7G3/1] Antibody (cGMP).