Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:25-1:75 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker Antibody Clone: 8G7G3/1
Host and Isotype: Mouse IgG1
Recommended Positive Control Sample: Lung
Cellular Localization of Antibody 8G7G3/1 Staining: Nuclear
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with a recombinant rat protein.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
Thyroid Transcription Factor-1, Thyroid and Lung Epithelial Marker Information for Pathologists
Summary:
Thyroid transcription factor, also called thyroid specific enhancer binding protein. 38 kDa nuclear protein that regulates transcription activity of thyroid (thyroglobulin, thyroperoxidase, sodium-iodide transport protein, calcitonin and MHC class I), lung (surfactant proteins A, B and C, Clara cell secretory protein) and diencephalon specific genes. Mutations cause pulmonary hypoplasia and neonatal death. Specificity depends on antibody clone used. Interpretation: nuclear stain.Common Uses By Pathologists:
Distinguish primary (TTF1+) vs. metastatic (usually TTF1-) lung carcinoma. Distinguish pulmonary adenocarcinoma (TTF1+) from squamous cell carcinoma (usually TTF1-). Pleural lung carcinoma (TTF1+) vs. mesothelioma (TTF1-). Pulmonary small cell carcinoma (TTF1+) vs. Merkel cell carcinoma (TTF1-). Microscopic (histologic) imagesLimitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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