Formalin-fixed, paraffin-embedded Rat Pancreas stained with TNF alpha Monoclonal Antibody (TNF76)
Formalin-fixed, paraffin-embedded Rat Pancreas stained with TNF alpha Monoclonal Antibody (TNF76)

Anti-TNF-alpha Antibody Clone TNF706

$ 429.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7124-MSM5
Species: Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7124-MSM5-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 7124-MSM5-P1
TNF-alpha General Information
Alternate Names
Tumor necrosis factor, TNF, TNFalpha, TNFa, TNF alpha, TNF?
Molecular Weight
17kDa
Chromosomal Location
6p21.33
Curated Database and Bioinformatic Data
Gene SymbolTNF
Entrez Gene ID7124
Ensemble Gene IDENSG00000232810, ENSG00000230108, ENSG00000223952, ENSG00000206439, ENSG00000228849, ENSG00000228978, ENSG00000228321, ENSG00000204490
RefSeq Protein Accession(s)NP_000585
RefSeq mRNA Accession(s)NM_000594
RefSeq Genomic Accession(s)NG_167244, NG_167248, NC_018917, NG_167246, NG_167247, NG_007462, NG_113891, NG_167245, NG_167249, NC_000006
UniProt ID(s)Q5STB3, P01375
UniGene ID(s)Q5STB3, P01375
HGNC ID(s)11892
Cosmic ID(s)TNF
KEGG Gene ID(s)hsa:7124
PharmGKB ID(s)PA435
General Description of TNF-alpha.
Tumor Necrosis Factor Alpha (TNF alpha) is a protein secreted by lipopolysaccharide-stimulated macrophages,, causes tumor necrosis when injected into tumor bearing mice. TNF alpha is believed to mediate pathogenic shock, tissue injury associated with endotoxemia. TNF alpha exists as a multimer of two, three, or five non-covalently linked units, but shows a single 17kDa band following SDS PAGE under non-reducing conditions. TNF alpha is closely related to the 25kDa protein Tumor Necrosis Factor beta (lymphotoxin), sharing the same receptors, cellular actions. TNF alpha causes cytolysis of certain transformed cells, being synergistic with interferon gamma in its cytotoxicity. Although it has little effect on many cultured normal human cells, TNF alpha appears to be directly toxic to vascular endothelial cells. Other actions of TNF alpha include stimulating growth of human fibroblasts, other cell lines, activating polymorphonuclear neutrophils, osteoclasts,, induction of interleukin 1, prostaglandin E2, collagenase production.

Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish Anti-TNF-alpha Antibody Product Attributes

Species: Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-paraffin) (2-4ug of antibody/ml for 30 min at RT)
Clonality: Monoclonal
Anti-TNF-alpha Antibody Clone: TNF706
Clone TNF706 Host and Isotype: Mouse IgM kappa
Anti-Human, Mouse, Rat, Rabbit, Cat, Canine, and Zebrafish TNF-alpha Positive Control Sample: HeLa, HL-60, or A431 cells. Macrophages in lymph node or Tonsil
Cellular Localization of Antibody Cytoplasmic, extracellular (secreted)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant N-terminal fragment of human TNF-?
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

TNF-alpha Previously Observed Antibody Staining Patterns

Limitations and Warranty

enQuire Bio's TNF-alpha Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-TNF-alpha Antibody Clone TNF706

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