Antibody (Suitable for clinical applications)
Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
| Specification | Recommendation |
|---|---|
| Recommended Dilution (Conc) | 1:50-1:100 |
| Pretreatment | EDTA Buffer pH 8.0 |
| Incubation Parameters | 30 min at Room Temperature |
Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.
Clonality: Monoclonal
Anti-TNF-alpha Antibody Clone: DBM15.28
Host and Isotype: Mouse IgM, kappa
Recommended Positive Control Sample: Histiocytoma, Colon, Pancreas
Cellular Localization of Antibody DBM15.28 Staining: Cytoplasmic and extracellular (secreted)
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant full-length human TNF-alpha protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.
TNF-alpha Information for Pathologists
Summary:
Also called cachectin. Initiates similar responses as lymphotoxin alpha. Secreted by macrophages and lipopolysaccharides or macrophage activating agents. Binds to 55kDa receptor on many cells throughout body. Has many biologic activities; initiate signals for cell proliferation and apoptosis; required for normal development and function of immune system; suppresses expression of lipoprotein lipase and anabolic enzymes in fat. In large boluses, modifies coagulation properties of endothelial cells, activates neutrophils, induces release of inflammatory cytokines (IL-1), causing cardiovascular collapse. In small amounts, causes symptoms of inflammatory response of bone resorption, fever, anemia, wasting. Both triggers apoptosis and activates NFKB which blocks apoptosis. TNF and IL-1 react with hypothalamic receptors, leading to stimulation of prostaglandin and leukotriene mediated pathways that reset brains thermoregulatory center.Limitations and Warranty
This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.
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