Immunohistochemical staining of TNF-alpha  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

TNF-alpha [DBM15.28] Antibody (cGMP).

$ 135.00$ 645.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD197
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgM, kappa

Product NumberDescriptionPrice
QIVD197-0.1ml Size: 0.1 ml, Format: Concentrate $ 158.00
QIVD197-0.5ml Size: 0.5 ml, Format: Concentrate $ 468.00
QIVD197-1ml Size: 1 ml, Format: Concentrate $ 648.00
QIVD197-6ml Size: 6 ml, Format: Predilute $ 288.00
QIVD197-25ml Size: 25 ml, Format: Predilute $ 728.00
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In Stock
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Datasheets and Documentation
Product Datasheet
Certificate of Analysis and Tags (Coming Soon)
Lot Number:

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SKU: QIVD197-0.1ml
TNF-alpha General Information
Alternate Names
Molecular Weight
25.6 kDa
Chromosomal Location
p21.33 [chr: 6] [chr_start: 31575565] [chr_end: 31578336] [strand: 1]; [chr: CHR_HSCHR6_MHC_SSTO_CTG1] [chr_start: 31566312] [chr_end: 31569081] [strand: 1]; [chr: CHR_HSCHR6_MHC_MCF_CTG1] [chr_start: 31651872] [chr_end: 31654641] [strand: 1]; [chr: CHR_HSCHR6_MHC_DBB_CTG1] [chr_start: 31557707] [chr_end: 31560476] [strand: 1]; [chr: CHR_HSCHR6_MHC_QBL_CTG1] [chr_start: 31565793] [chr_end: 31568564] [strand: 1]; [chr: CHR_HSCHR6_MHC_APD_CTG1] [chr_start: 31643520] [chr_end: 31645322] [strand: 1]; [chr: CHR_HSCHR6_MHC_MANN_CTG1] [chr_start: 31615015] [chr_end: 31617784] [strand: 1]; [chr: CHR_HSCHR6_MHC_COX_CTG1] [chr_start: 31562973] [chr_end: 31565742] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolTNF
Entrez Gene ID7124
RefSeq Protein Accession(s)NP_000585
RefSeq mRNA Accession(s); NM_000594
RefSeq Genomic Accession(s)NT_167244; NT_167248; NT_167246; NT_167247; NG_007462; NT_113891; NT_167245; NT_167249; NC_000006
UniProt ID(s)P01375
PharmGKB ID(s)PA435
KEGG Gene ID(s)hsa:7124
Associated Diseases (KEGG IDs)Psoriatic arthritis (PSORAS) [MIM:607507]: An inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoid like pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis). {ECO:0000269|PubMed:12746914}. Disease susceptibility is associated with variations affecting the gene represented in this entry.
General Description of TNF-alpha .
This MAb recognizes human 17-26kDa protein, which is identified as cytokine TNF-alpha (Tumor Necrosis Factor-alpha). Monomeric human TNF-alpha is a 157 amino acid protein (non-glycosylated) with a reported molecular weight of 17 kDa and can be expressed as a free molecule, also TNF-alpha is generated as a precursor form called transmembrane TNF-alpha can be expressed as a cell surface type II polypeptide consisting of 233 amino acid residues molecular weight 26 kDa. TNF-alpha is an important cell-signaling component of the immune system. It is a protein secreted by LPS stimulated macrophages, and causes tumor necrosis when injected into tumor bearing mice. TNF-alpha is currently being evaluated in treatment of certain cancers and AIDS Related Complex.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:50-1:100
Pretreatment EDTA Buffer pH 8.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-TNF-alpha Antibody Clone: DBM15.28
Host and Isotype: Mouse IgM, kappa
Recommended Positive Control Sample: Histiocytoma, Colon, Pancreas
Cellular Localization of Antibody DBM15.28 Staining: Cytoplasmic and extracellular (secreted)
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: Recombinant full-length human TNF-alpha protein
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

TNF-alpha Information for Pathologists

Summary:

Also called cachectin. Initiates similar responses as lymphotoxin alpha. Secreted by macrophages and lipopolysaccharides or macrophage activating agents. Binds to 55kDa receptor on many cells throughout body. Has many biologic activities; initiate signals for cell proliferation and apoptosis; required for normal development and function of immune system; suppresses expression of lipoprotein lipase and anabolic enzymes in fat. In large boluses, modifies coagulation properties of endothelial cells, activates neutrophils, induces release of inflammatory cytokines (IL-1), causing cardiovascular collapse. In small amounts, causes symptoms of inflammatory response of bone resorption, fever, anemia, wasting. Both triggers apoptosis and activates NFKB which blocks apoptosis. TNF and IL-1 react with hypothalamic receptors, leading to stimulation of prostaglandin and leukotriene mediated pathways that reset brains thermoregulatory center.

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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TNF-alpha [DBM15.28] Antibody (cGMP).