Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).
Formalin-fixed, paraffin-embedded human Melanoma stained with Topo I, MT Monoclonal Antibody (TOP1MT/488).Formalin-fixed, paraffin-embedded human Pancreas stained with  Topo I, MT Monoclonal Antibody (TOP1MT/488).

Anti-Topoisomerase I, Mitochondrial Antibody Clone TOP1MT/488

$ 298.00
Please Select Product Options Below To View The Catalog Number.

SKU: 116447-MSM1
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2b kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
116447-MSM1-P1 Size: 100 ug, Tag: Unconjugated
Shipping Information
In Stock: 1-2 Day Lead Time.
Flat Rate Shipping Anywhere in the US: $45
Datasheets and Documentation
Product Datasheet
SKU: 116447-MSM1-P1
Topoisomerase I, Mitochondrial General Information
Alternate Names
Aspartate aminotransferase, Cytoplasmic Aspartate Aminotransferase
Molecular Weight
70kDa
Chromosomal Location
8q24.3
Curated Database and Bioinformatic Data
Gene SymbolTOP1MT
Entrez Gene ID116447
Ensemble Gene IDENSG00000184428
RefSeq Protein Accession(s)XP_005250838, NP_443195, NP_001245375, NP_001245376
RefSeq mRNA Accession(s)XM_005250781, NM_052963, NM_001258446, NM_001258447
RefSeq Genomic Accession(s)NC_000008, NC_018919
UniProt ID(s)Q969P6, E5KMK7, B4DYD2
UniGene ID(s)Q969P6, E5KMK7, B4DYD2
HGNC ID(s)29787
Cosmic ID(s)TOP1MT
KEGG Gene ID(s)hsa:116447
PharmGKB ID(s)PA134922772
General Description of Topoisomerase I, Mitochondrial.
DNA topoisomerases are nuclear enzymes that regulate the topological structure of DNA in eukaryotic cells by transiently breaking, rejoining DNA strands. Due to their roles in DNA replication, recombination,, transcription, DNA topoisomerases have been identified as targets of numerous anticancer drugs. Mitochondrial Topo I (DNA topoisomerase I, mitochondrial) is a 601 amino acid protein that primarily acts to relieve DNA strain that may occur during duplication of mitochondrial DNA. As a type IB topoisomerase, mitochondrial Topo I requires a divalent metal, either, calcium or magnesium, as well as an alkaline pH for optimal activity.

Human Anti-Topoisomerase I, Mitochondrial Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Topoisomerase I, Mitochondrial Antibody Clone: TOP1MT/488
Clone TOP1MT/488 Host and Isotype: Mouse IgG2b kappa
Anti-Human Topoisomerase I, Mitochondrial Positive Control Sample: A431 cells. Heart, Skeletal muscle, brain or fetal liver.
Cellular Localization of Antibody Cytoplasmic (Mitochondria)
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human TOP1MT protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Topoisomerase I, Mitochondrial Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TOP1MT antibody staining is expected to be primarily localized to the mitochondria.

Observed Antibody Staining Data By Tissue Type:

Variations in Topoisomerase I, Mitochondrial antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the appendix, hematopoietic cells in the bone marrow, glandular cells in the breast, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, Purkinje cells in the cerebellum, neuronal cells in the cerebral cortex, squamous epithelial cells in the cervix, uterine, glandular cells in the colon, duodenum, endometrium, epididymis, fallopian tube and gallbladder, myocytes in heart muscle, neuronal cells in the hippocampus, cells in the tubules in kidney, hepatocytes in liver, macrophages in lung, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, small intestine and stomach, cells in the seminiferous ducts in testis, glandular cells in the thyroid gland, germinal center cells in the tonsil, non-germinal center cells in the tonsil and urothelial cells in the urinary bladder. More moderate antibody staining intensity was present in glandular cells in the appendix, hematopoietic cells in the bone marrow, glandular cells in the breast, myoepithelial cells in the breast, neuronal cells in the caudate nucleus, Purkinje cells in the cerebellum, neuronal cells in the cerebral cortex, squamous epithelial cells in the cervix, uterine, glandular cells in the colon, duodenum, endometrium, epididymis, fallopian tube and gallbladder, myocytes in heart muscle, neuronal cells in the hippocampus, cells in the tubules in kidney, hepatocytes in liver, macrophages in lung, pneumocytes in lung, respiratory epithelial cells in the nasopharynx, exocrine glandular cells in the pancreas, glandular cells in the parathyroid gland, decidual cells in the placenta, trophoblastic cells in the placenta, glandular cells in the prostate, small intestine and stomach, cells in the seminiferous ducts in testis, glandular cells in the thyroid gland, germinal center cells in the tonsil, non-germinal center cells in the tonsil and urothelial cells in the urinary bladder. Low, but measureable presence of Topoisomerase I, Mitochondrial could be seen in cells in the endometrial stroma in endometrium, glial cells in the hippocampus, germinal center cells in the lymph node, Langerhans in skin, melanocytes in skin, smooth muscle cells in the smooth muscle, adipocytes in mesenchymal tissue and cells in the white pulp in spleen. We were unable to detect Topoisomerase I, Mitochondrial in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Topoisomerase I, Mitochondrial expression as measured by anti-Topoisomerase I, Mitochondrial antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity ++ + ++ +++ +++ ++ ++ +++ ++ ++ ++ ++ ++ ++ + ++ ++ ++ ++ ++
TOP1MT Variability ++ ++ ++ ++ ++ ++ +++ ++ ++ + ++ ++ + + ++ ++ ++ + ++ ++

Limitations and Warranty

enQuire Bio's Topoisomerase I, Mitochondrial Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Topoisomerase I, Mitochondrial Antibody Clone TOP1MT/488

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