Human, Cow, Pig, Rabbit, and Mouse Anti-Transgelin Antibody Product Attributes
Species: Human, Cow, Pig, Rabbit, and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-Transgelin Antibody Clone: TAGLN/247
Clone TAGLN/247 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Cow, Pig, Rabbit, and Mouse Transgelin Positive Control Sample: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.
Cellular Localization of Antibody
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant full-length human transgelin (TAGLN) protein.
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
Transgelin Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:Anti-TAGLN antibody staining is expected to be primarily localized to the cytosol, microtubules and mitochondria. There is variability in either the signal strength or the localization of signal in cytosol from cell to cell.
Observed Antibody Staining Data By Tissue Type:Variations in Transgelin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. Low, but measureable presence of Transgelin could be seen inovarian stroma cells in the ovary. We were unable to detect Transgelin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Limitations and Warranty
enQuire Bio's Transgelin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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