Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with Transgelin Monoclonal Antibody (TAGLN/247)

Anti-Transgelin Antibody Clone TAGLN/247

Name: Anti-Transgelin Antibody Clone TAGLN/247
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 488.00

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SKU: 6876-MSM1
Species: Human, Cow, Pig, Rabbit, and Mouse
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
6876-MSM1-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

Certificate of Analysis and Tags (Coming Soon)

SKU: 6876-MSM1-P1

Transgelin General Information
Alternate Names
Transgelin, TAGLN
Molecular Weight
22kDa
Chromosomal Location
11q23.2
Curated Database and Bioinformatic Data
Gene Symbol	TAGLN
Entrez Gene ID	6876
Ensemble Gene ID	ENSG00000149591
RefSeq Protein Accession(s)	NP_001001522, NP_003177
RefSeq mRNA Accession(s)	NM_003186, NM_001001522
RefSeq Genomic Accession(s)	NC_000011, NC_018922
UniProt ID(s)	Q5U0D2, Q01995
UniGene ID(s)	Q5U0D2, Q01995
HGNC ID(s)	11553
Cosmic ID(s)	TAGLN
KEGG Gene ID(s)	hsa:6876
PharmGKB ID(s)	PA36324
General Description of Transgelin.
This MAb recognizes a 22kDa protein, identified as Transgelin, also designated SM22-alpha. It may cross-react with SM22-beta. Transgelin is expressed abundantly in smooth muscle cells. The human transgelin gene encodes a 201 amino acid protein that contains nuclear factor-binding motifs known to regulate transcription in smooth muscle. During embryogenesis, transgelin is expressed in smooth, cardiac, skeletal muscle, but is restricted during late fetal development, adulthood to all vascular, visceral smooth muscle cells, low levels of expression in heart. Transgelin is down regulated in several transformed cell lines, indicating that a reduction of transgelin expression may be an early indicator of the onset of transformation. Transgelin also binds Actin, causing Actin fibers to gel within minutes of binding. Binding of transgelin to Actin occurs at a ratio of 1:6 Actin monomers.

Human, Cow, Pig, Rabbit, and Mouse Anti-Transgelin Antibody Product Attributes

Species: Human, Cow, Pig, Rabbit, and Mouse

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-Transgelin Antibody Clone: TAGLN/247

Clone TAGLN/247 Host and Isotype: Mouse IgG1 kappa

Anti-Human, Cow, Pig, Rabbit, and Mouse Transgelin Positive Control Sample: U-2 OS, Hs68 or WI 38 cells. Colon carcinoma.

Cellular Localization of Antibody Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Recombinant full-length human transgelin (TAGLN) protein.

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

Transgelin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TAGLN antibody staining is expected to be primarily localized to the cytosol, microtubules and mitochondria. There is variability in either the signal strength or the localization of signal in cytosol from cell to cell.

Observed Antibody Staining Data By Tissue Type:

Variations in Transgelin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. More moderate antibody staining intensity was present in myoepithelial cells in the breast and smooth muscle cells in the smooth muscle. Low, but measureable presence of Transgelin could be seen inovarian stroma cells in the ovary. We were unable to detect Transgelin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's Transgelin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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