Human Anti-TRIM29 Antibody Product Attributes
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-TRIM29 Antibody Clone: TRIM29/1041
Clone TRIM29/1041 Host and Isotype: Mouse IgG2a kappa
Anti-Human TRIM29 Positive Control Sample: A431 cells. Tonsil or Squamous cell carcinoma.
Cellular Localization of Antibody Cytoplasmic & Cell Surface
Buffer and Stabilizer: 1mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant fragment (126 Amino acid residues between aa 1-200) of human TRIM29 protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.
TRIM29 Previously Observed Antibody Staining Patterns
Observed Subcellular, Organelle Specific Staining Data:
Anti-TRIM29 antibody staining is expected to be primarily localized to the intermediate filaments and nucleoplasm.Observed Antibody Staining Data By Tissue Type:
Variations in TRIM29 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in squamous epithelial cells in the cervix, uterine and esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, melanocytes in skin, epidermal cells in the skin and squamous epithelial cells in the tonsil and vagina. More moderate antibody staining intensity was present in squamous epithelial cells in the cervix, uterine and esophagus, respiratory epithelial cells in the nasopharynx, squamous epithelial cells in the oral mucosa, keratinocytes in skin, melanocytes in skin, epidermal cells in the skin and squamous epithelial cells in the tonsil and vagina. Low, but measureable presence of TRIM29 could be seen inmyoepithelial cells in the breast, glandular cells in the epididymis and fallopian tube, pneumocytes in lung, decidual cells in the placenta and trophoblastic cells in the placenta. We were unable to detect TRIM29 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.Observed Antibody Staining Data By Tissue Disease Status:
Tissues from cancer patients, for instance, have their own distinct pattern of TRIM29 expression as measured by anti-TRIM29 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.| Sample Type | breast cancer | carcinoid | cervical cancer | colorectal cancer | endometrial cancer | glioma | head and neck cancer | liver cancer | lung cancer | lymphoma | melanoma | ovarian cancer | pancreatic cancer | prostate cancer | renal cancer | skin cancer | stomach cancer | testicular cancer | thyroid cancer | urothelial cancer |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Signal Intensity | - | - | ++ | + | - | - | ++ | + | ++ | - | - | - | - | - | - | +++ | - | - | - | ++ |
| TRIM29 Variability | + | + | ++ | ++ | + | + | ++ | +++ | ++ | + | + | ++ | ++ | + | + | ++ | + | + | + | +++ |
Limitations and Warranty
enQuire Bio's TRIM29 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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