Human, Mouse, Rat, and Broad Species Reactivity Anti-TTF-1 / NKX2.1 Antibody Product Attributes
Species: Human, Mouse, Rat, and Broad Species Reactivity
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Anti-TTF-1 / NKX2.1 Antibody Clone: 8G7G3/1
Clone 8G7G3/1 Host and Isotype: Mouse IgG1 kappa
Anti-Human, Mouse, Rat, and Broad Species Reactivity TTF-1 / NKX2.1 Positive Control Sample: MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.
Cellular Localization of Antibody <8G7G3/1 Staining: Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Rat full length TTF-1 recombinant protein
Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.
TTF-1 / NKX2.1 Previously Observed Antibody Staining Patterns
Observed Antibody Staining Data By Tissue Type:Variations in TTF-1 / NKX2.1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the thyroid gland. More moderate antibody staining intensity was present in glandular cells in the thyroid gland. Low, but measureable presence of TTF-1 / NKX2.1 could be seen inrespiratory epithelial cells in the bronchus, macrophages in lung and respiratory epithelial cells in the nasopharynx. We were unable to detect TTF-1 / NKX2.1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.
Observed Antibody Staining Data By Tissue Disease Status:Tissues from cancer patients, for instance, have their own distinct pattern of TTF-1 / NKX2.1 expression as measured by anti-TTF-1 / NKX2.1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.
|Sample Type||breast cancer||carcinoid||cervical cancer||colorectal cancer||endometrial cancer||glioma||head and neck cancer||liver cancer||lung cancer||lymphoma||melanoma||ovarian cancer||pancreatic cancer||prostate cancer||renal cancer||skin cancer||stomach cancer||testicular cancer||thyroid cancer||urothelial cancer|
Limitations and Warranty
enQuire Bio's TTF-1 / NKX2.1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.
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