Formalin-fixed, paraffin-embedded human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/69)
Formalin-fixed, paraffin-embedded human Thyroid stained with TTF-1 Monoclonal Antibody (8G7G3/1 + NX2.1/69)

Anti-TTF-1 / NKX2.1 Antibody Clone 8G7G3/1 + NX2.1/690

$ 298.00
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SKU: 7080-MSM3
Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7080-MSM3-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 7080-MSM3-P1
TTF-1 / NKX2.1 General Information
Alternate Names
NK2 homeobox 1, NKX2-1, thyroid transcription factor 1, TTF-1
Molecular Weight
40kDa
Chromosomal Location
14q13.3
Curated Database and Bioinformatic Data
Gene SymbolNKX2-1
Entrez Gene ID7080
Ensemble Gene IDENSG00000136352
RefSeq Protein Accession(s)NP_001073136, NP_003308
RefSeq mRNA Accession(s)NM_003317 NM_001079668
RefSeq Genomic Accession(s)NC_018925, NG_013365, NC_000014
UniProt ID(s)P43699
UniGene ID(s)P43699
HGNC ID(s)11825
Cosmic ID(s)NKX2-1
KEGG Gene ID(s)hsa:7080
PharmGKB ID(s)PA36531
General Description of TTF-1 / NKX2.1.
Recognizes a protein of 40kDa, identified as Thyroid transcription factor-1 (TTF-1). TTF-1 is a member of the NKx2 family of homeodomain transcription factors. It is expressed in epithelial cells of the thyroid gland, the lung. Nuclei from liver, stomach, pancreas, small intestine, colon, kidney, breast, skin, testes, pituitary, prostate,, adrenal glands are unreactive. Anti-TTF-1 is useful in differentiating primary adenocarcinoma of the lung from metastatic carcinomas originating in the breast, mediastinal germ cell tumors,, malignant mesothelioma. It can also be used to differentiate small cell lung carcinoma from lymphoid infiltrates.Loss of TTF-1 expression in non-small cell lung carcinoma has been associated with aggressive behavior of such neoplasms. TTF-1 reactivity is also seen in thyroid malignancies.

Human, Mouse, and Rat Anti-TTF-1 / NKX2.1 Antibody Product Attributes

Species: Human, Mouse, and Rat
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-TTF-1 / NKX2.1 Antibody Clone: 8G7G3/1 + NX2.1/690
Clone 8G7G3/1 + NX2.1/690 Host and Isotype: Mouse IgG
Anti-Human, Mouse, and Rat TTF-1 / NKX2.1 Positive Control Sample: MAD109, MLE-15, H441-4, or H345 cells. Normal thyroid or lung.
Cellular Localization of Antibody Nuclear
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Rat full length TTF-1 recombinant protein (8G7G3/1); Recombinant TTF-1 protein (NX2.1/690)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

TTF-1 / NKX2.1 Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in TTF-1 / NKX2.1 antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the thyroid gland. More moderate antibody staining intensity was present in glandular cells in the thyroid gland. Low, but measureable presence of TTF-1 / NKX2.1 could be seen inrespiratory epithelial cells in the bronchus, macrophages in lung and respiratory epithelial cells in the nasopharynx. We were unable to detect TTF-1 / NKX2.1 in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of TTF-1 / NKX2.1 expression as measured by anti-TTF-1 / NKX2.1 antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - ++ - - - - - - - - - ++ -
NKX2-1 Variability + + + + + + + ++ ++ + + + + + + + + + ++ +

Limitations and Warranty

enQuire Bio's TTF-1 / NKX2.1 Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-TTF-1 / NKX2.1 Antibody Clone 8G7G3/1 + NX2.1/690

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