Formalin-fixed, paraffin-embedded human Melanoma stained with Tyrosinase Monoclonal Antibody (SPM36).
Formalin-fixed, paraffin-embedded human Melanoma stained with Tyrosinase Monoclonal Antibody (SPM36).

Anti-Tyrosinase Antibody Clone SPM360

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7299-MSM1X
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG2a kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7299-MSM1X-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
Flat Rate Shipping Anywhere in the US: $45
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SKU: 7299-MSM1X-P1
Tyrosinase General Information
Alternate Names
Tyrosinase, Tumor Rejection Antigen AB
Molecular Weight
70-80kDa
Chromosomal Location
11q14.3
Curated Database and Bioinformatic Data
Gene SymbolTYR
Entrez Gene ID7299
Ensemble Gene IDENSG00000077498
RefSeq Protein Accession(s)XP_011541272, NP_000363
RefSeq mRNA Accession(s)NM_000372, XM_011542970,
RefSeq Genomic Accession(s)NC_000011, NG_008748, NC_018922
UniProt ID(s)P14679, L8B082
UniGene ID(s)P14679, L8B082
HGNC ID(s)12442
Cosmic ID(s)TYR
KEGG Gene ID(s)hsa:7299
PharmGKB ID(s)PA37095
General Description of Tyrosinase.
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1, tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa),, the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes, melanomas.

Human Anti-Tyrosinase Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Tyrosinase Antibody Clone: SPM360
Clone SPM360 Host and Isotype: Mouse IgG2a kappa
Anti-Human Tyrosinase Positive Control Sample: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant tyrosinase protein
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Tyrosinase Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TYR antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Tyrosinase antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in melanocytes in skin. More moderate antibody staining intensity was present in melanocytes in skin. Low, but measureable presence of Tyrosinase could be seen in. We were unable to detect Tyrosinase in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Tyrosinase expression as measured by anti-Tyrosinase antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - - - - - - - - - +++ - - - - - - - - -
TYR Variability + + + + + + + + + + + + + + + + + + + +

Limitations and Warranty

enQuire Bio's Tyrosinase Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Tyrosinase Antibody Clone SPM360

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