Formalin-fixed, paraffin-embedded Melanoma stained with Tyrosinase Monoclonal Antibody (T311 + OCA1/812).

Anti-Tyrosinase Antibody Clone T311 + OCA1/812

Name: Anti-Tyrosinase Antibody Clone T311 + OCA1/812
Brand: enQuire Bio
Price: 429 USD
Availability: InStock

$ 429.00

Please Select Product Options Below To View The Catalog Number.

SKU: 7299-MSM3
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG
Mass Spec Validated?: Not MS Validated

Product Number	Description	Price
7299-MSM3-P1	Size: 100 ug, Tag: Unconjugated

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Datasheets and Documentation

Product Datasheet

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SKU: 7299-MSM3-P1

Tyrosinase General Information
Alternate Names
Tyrosinase, Tumor Rejection Antigen AB
Molecular Weight
70-80kDa
Chromosomal Location
11q14.3
Curated Database and Bioinformatic Data
Gene Symbol	TYR
Entrez Gene ID	7299
Ensemble Gene ID	ENSG00000077498
RefSeq Protein Accession(s)	XP_011541272, NP_000363
RefSeq mRNA Accession(s)	NM_000372, XM_011542970,
RefSeq Genomic Accession(s)	NC_000011, NG_008748, NC_018922
UniProt ID(s)	P14679, L8B082
UniGene ID(s)	P14679, L8B082
HGNC ID(s)	12442
Cosmic ID(s)	TYR
KEGG Gene ID(s)	hsa:7299
PharmGKB ID(s)	PA37095
General Description of Tyrosinase.
Recognizes a cluster of proteins between 70-80kDa, identified as tyrosinase. Occasionally a minor band at 55kDa is also detected. This MAb shows no cross-reaction with MAGE-1, tyrosinase-related protein 1, TRP-1/gp75. Tyrosinase is a copper-containing metalloglycoprotein that catalyzes several steps in the melanin pigment biosynthetic pathway; the hydroxylation of tyrosine to L-3,4-dihydroxy-phenylalanine (dopa),, the subsequent oxidation of dopa to dopaquinone. Mutations of the tyrosinase gene occur in various forms of albinism. Tyrosinase is one of the targets for cytotoxic T-cell recognition in melanoma patients. Staining of melanomas with this MAb shows tyrosinase in melanotic as well as amelanotic variants. This MAb is a useful marker for melanocytes, melanomas.

Human Anti-Tyrosinase Antibody Product Attributes

Species: Human

Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).

Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (1-2ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1ug of antibody/ml for 30 minutes at RT)

Clonality: Monoclonal

Anti-Tyrosinase Antibody Clone: T311 + OCA1/812

Clone T311 + OCA1/812 Host and Isotype: Mouse IgG

Anti-Human Tyrosinase Positive Control Sample: SK-MEL-13, SK-MEL-19, SK-MEL-30, SK-MEL-37 cells or Melanoma.

Cellular Localization of Antibody Cytoplasmic

Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.

Antibody Concentration: 200ug/ml

Antibody Purification Method:Protein A/G Purified

Immunogen: Recombinant tyrosinase protein (T311); Recombinant human TYR protein (OCA1/812)

Storage Conditions: Store at 2 to 8Â° C (refrigerate). Stable for 24 months when properly stored.

Tyrosinase Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-TYR antibody staining is expected to be primarily localized to the vesicles.

Observed Antibody Staining Data By Tissue Type:

Variations in Tyrosinase antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in melanocytes in skin. More moderate antibody staining intensity was present in melanocytes in skin. Low, but measureable presence of Tyrosinase could be seen in. We were unable to detect Tyrosinase in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Tyrosinase expression as measured by anti-Tyrosinase antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type	breast cancer	carcinoid	cervical cancer	colorectal cancer	endometrial cancer	glioma	head and neck cancer	liver cancer	lung cancer	lymphoma	melanoma	ovarian cancer	pancreatic cancer	prostate cancer	renal cancer	skin cancer	stomach cancer	testicular cancer	thyroid cancer	urothelial cancer
Signal Intensity	-	-	-	-	-	-	-	-	-	-	+++	-	-	-	-	-	-	-	-	-
TYR Variability	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+	+

Limitations and Warranty

enQuire Bio's Tyrosinase Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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