Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1325).
Formalin-fixed, paraffin-embedded human Colon stained with Villin Monoclonal Antibody (VIL1/1325).Western Blot of A549, HepG2 & HCT116 Cell Lysates with Villin Monoclonal Antibody (VIL1/1325).

Anti-Villin Antibody Clone VIL1/1325

$ 228.00$ 518.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7429-MSM2
Species: Human
Tested Applications: Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7429-MSM2-P0 Size: 20 ug, Tag: Unconjugated $ 199.00
7429-MSM2-P1 Size: 100 ug, Tag: Unconjugated $ 429.00
7429-MSM2-P2 Size: 200 ug, Tag: Unconjugated $ 459.00
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In Stock.
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SKU: 7429-MSM2-P0
Villin General Information
Alternate Names
Villin-1, VIL1
Molecular Weight
93kDa
Chromosomal Location
2q35
Curated Database and Bioinformatic Data
Gene SymbolVIL1
Entrez Gene ID7429
Ensemble Gene IDENSG00000127831
RefSeq Protein Accession(s)NP_009058
RefSeq mRNA Accession(s)NM_007127
RefSeq Genomic Accession(s)NC_000002, NC_018913
UniProt ID(s)P09327, Q53F91
UniGene ID(s)P09327, Q53F91
HGNC ID(s)12690
Cosmic ID(s)VIL1
KEGG Gene ID(s)hsa:7429
PharmGKB ID(s)PA37309
General Description of Villin.
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.

Human Anti-Villin Antibody Product Attributes

Species: Human
Tested Applications: Immunohistochemistry (IHC).
Clonality: Monoclonal
Anti-Villin Antibody Clone: VIL1/1325
Clone VIL1/1325 Host and Isotype: Mouse IgG1 kappa
Anti-Human Villin Positive Control Sample: A549, HepG2 and HCT116 cells. Colon or Rectum.
Cellular Localization of Antibody Cytoplasmic ;Cell Surface
Buffer and Stabilizer: 10mM PBS with 0.05% BSA and 0.05% Azide
Antibody Concentration: 200 ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human Villin fragment of 133 amino acid residues (aa179-311)
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Villin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-VIL1 antibody staining is expected to be primarily localized to the plasma membrane.

Observed Antibody Staining Data By Tissue Type:

Variations in Villin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in glandular cells in the colon, duodenum, gallbladder, rectum, small intestine. More moderate antibody staining intensity was present in glandular cells in the colon, duodenum, gallbladder, rectum, small intestine. Low, but measureable presence of Villin could be seen inhepatocytes in liver and keratinocytes in skin. We were unable to detect Villin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Villin expression as measured by anti-Villin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - ++ - ++ - - - - - - - - - - - - ++ - - -
VIL1 Variability + ++ + ++ + + + + + + + + ++ + ++ + ++ + + +

Limitations and Warranty

enQuire Bio's Villin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Villin Antibody Clone VIL1/1325

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