Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).
Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (LN-6).

Anti-Vimentin Antibody Clone LN-6

$ 488.00
Please Select Product Options Below To View The Catalog Number.

SKU: 7431-MSM2
Species: Human, Cat, Cow, Pig, Rat, Rabbit, Mouse, and Sheep
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgM
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7431-MSM2-P1 Size: 100 ug, Tag: Unconjugated
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SKU: 7431-MSM2-P1
Vimentin General Information
Alternate Names
Vimentin, Anti-Vimentin
Molecular Weight
57-60kDa
Chromosomal Location
10p12.33
Curated Database and Bioinformatic Data
Gene SymbolVIM
Entrez Gene ID7431
Ensemble Gene IDENSG00000026025
RefSeq Protein Accession(s)NP_003371, XP_006717563
RefSeq mRNA Accession(s)XM_006717500, NM_003380
RefSeq Genomic Accession(s)NC_018921, NC_000010, NG_012413
UniProt ID(s)P08670, V9HWE1
UniGene ID(s)P08670, V9HWE1
HGNC ID(s)12692
Cosmic ID(s)VIM
KEGG Gene ID(s)hsa:7431
PharmGKB ID(s)PA37311
General Description of Vimentin.
This MAb reacts with a 58kDa protein identified as vimentin. It reacts with a non-hematopoietic epitope of vimentin, shows no cross-reaction with other closely related intermediate filament proteins (IFPs) such as desmin, keratin, neurofilament,, glial fibrillary acid protein.Vimentin is ubiquitously expressed in mesenchymal cells such as fibroblasts, smooth muscle cells,, endothelium. Antibody against vimentin is useful as part of an antibody panel for differential diagnosis of tumors of unknown origin. This antibody does not react with leukocyte common antigen-positive tissues such as lymphomas, leukemias.

Human, Cat, Cow, Pig, Rat, Rabbit, Mouse, and Sheep Anti-Vimentin Antibody Product Attributes

Species: Human, Cat, Cow, Pig, Rat, Rabbit, Mouse, and Sheep
Tested Applications: Flow Cytometry, Immunofluorescence, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Immunohistochemistry (IHC) (Formalin-fixed) (1-2ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-Vimentin Antibody Clone: LN-6
Clone LN-6 Host and Isotype: Mouse IgM
Anti-Human, Cat, Cow, Pig, Rat, Rabbit, Mouse, and Sheep Vimentin Positive Control Sample: Jurkat cells, Sarcomas, Melanomas
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Human thymic nuclear extract
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

Vimentin Previously Observed Antibody Staining Patterns

Observed Subcellular, Organelle Specific Staining Data:

Anti-VIM antibody staining is expected to be primarily localized to the intermediate filaments.

Observed Antibody Staining Data By Tissue Type:

Variations in Vimentin antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, adipocytes in breast, endothelial cells in the cerebral cortex and colon, glandular cells in the epididymis and fallopian tube, cells in the glomeruli in kidney, macrophages in lung, pneumocytes in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, ovarian stroma cells in the ovary, glandular cells in the seminal vesicle, fibroblasts in skin, Langerhans in skin, melanocytes in skin, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland, germinal center cells in the tonsil and non-germinal center cells in the tonsil. More moderate antibody staining intensity was present in lymphoid tissue in appendix, hematopoietic cells in the bone marrow, adipocytes in breast, endothelial cells in the cerebral cortex and colon, glandular cells in the epididymis and fallopian tube, cells in the glomeruli in kidney, macrophages in lung, pneumocytes in lung, germinal center cells in the lymph node, non-germinal center cells in the lymph node, ovarian stroma cells in the ovary, glandular cells in the seminal vesicle, fibroblasts in skin, Langerhans in skin, melanocytes in skin, adipocytes in mesenchymal tissue, fibroblasts in mesenchymal tissue, peripheral nerve in mesenchymal tissue, cells in the red pulp in spleen, cells in the seminiferous ducts in testis, Leydig cells in the testis, glandular cells in the thyroid gland, germinal center cells in the tonsil and non-germinal center cells in the tonsil. Low, but measureable presence of Vimentin could be seen inglandular cells in the adrenal gland, respiratory epithelial cells in the bronchus, glial cells in the cerebral cortex, cells in the endometrial stroma in endometrium, glial cells in the hippocampus, bile duct cells in the liver, respiratory epithelial cells in the nasopharynx, follicle cells in the ovary, decidual cells in the placenta and squamous epithelial cells in the vagina. We were unable to detect Vimentin in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Observed Antibody Staining Data By Tissue Disease Status:

Tissues from cancer patients, for instance, have their own distinct pattern of Vimentin expression as measured by anti-Vimentin antibody immunohistochemical staining. The average level of expression by tumor is summarized in the table below. The variability row represents patient to patient variability in IHC staining.

Sample Type breast cancer carcinoid cervical cancer colorectal cancer endometrial cancer glioma head and neck cancer liver cancer lung cancer lymphoma melanoma ovarian cancer pancreatic cancer prostate cancer renal cancer skin cancer stomach cancer testicular cancer thyroid cancer urothelial cancer
Signal Intensity - - + - ++ - ++ ++ ++ +++ +++ + ++ - +++ ++ ++ ++ +++ ++
VIM Variability + + ++ ++ ++ ++ ++ ++ + ++ + ++ + ++ + ++ + ++ + ++

Limitations and Warranty

enQuire Bio's Vimentin Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-Vimentin Antibody Clone LN-6

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