Immunohistochemical staining of Vimentin  of human FFPE tissue followed by incubation with HRP labeled secondary and development with DAB substrate.

Vimentin [V9] Antibody (cGMP).

$ 128.00$ 408.00
Please Select Product Options Below To View The Catalog Number.

SKU: QIVD211
Species: Human
Tested Applications: IHC [IVD]
Available Conjugates:
Isotype: Mouse IgG1, kappa

Product NumberDescriptionPrice
QIVD211-0.1ml Size: 0.1 ml, Format: Concentrate $ 128.00
QIVD211-0.5ml Size: 0.5 ml, Format: Concentrate $ 238.00
QIVD211-1ml Size: 1 ml, Format: Concentrate $ 408.00
QIVD211-6ml Size: 6 ml, Format: Predilute $ 198.00
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Datasheets and Documentation
Product Datasheet
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SKU: QIVD211-0.1ml
Vimentin General Information
Alternate Names
Molecular Weight
53.7 kDa
Chromosomal Location
p13 [chr: 10] [chr_start: 17228241] [chr_end: 17237593] [strand: 1]
Curated Database and Bioinformatic Data
Gene SymbolVIM
Entrez Gene ID7431
RefSeq Protein Accession(s)XP_006717563; NP_003371
RefSeq mRNA Accession(s); XM_006717500; NM_003380
RefSeq Genomic Accession(s)NC_000010; NG_012413
UniProt ID(s)P08670
PharmGKB ID(s)PA37311
KEGG Gene ID(s)hsa:7431
Associated Diseases (KEGG IDs)Cataract 30, multiple types (CTRCT30) [MIM:116300]: An opacification of the crystalline lens of the eye that frequently results in visual impairment or blindness. Opacities vary in morphology, are often confined to a portion of the lens, and may be static or progressive. In general, the more posteriorly located and dense an opacity, the greater the impact on visual function. {ECO:0000269|PubMed:19126778, ECO:0000269|PubMed:26694549, ECO:0000269|PubMed:28450710}. The disease is caused by mutations affecting the gene represented in this entry.
General Description of Vimentin .
This antibody reacts with the 57 kDa intermediate filament protein present in the cells of mesenchymal origin. The antibody does not show any cross-reactivity with other intermediate filament proteins. However, it shows a broad interspecies cross-reactivity.

Antibody (Suitable for clinical applications)

Sample Type: FFPE Patient Samples.
Tested Applications: IHC. Approved for In Vitro Diagnostic Procedures on FFPE tissues. For tissue collection recommendations, please see datasheet sent with product.
Application Notes
Specification Recommendation
Recommended Dilution (Conc) 1:25-1:50
Pretreatment Citrate Buffer pH 6.0
Incubation Parameters 30 min at Room Temperature

Prior to use, inspect vial for the presence of any precipitate or other unusual physical properties. These can indicate that the antibody has degraded and is no longer suitable for patient samples. Please run positive and negative controls simultaneously with all patient samples to account and control for errors in laboratory procedure. Use of methods or materials not recommended by enQuire Bio including change to dilution range and detection system should be routinely validated by the user.

Clonality: Monoclonal
Anti-Vimentin Antibody Clone: V9
Host and Isotype: Mouse IgG1, kappa
Recommended Positive Control Sample: Sarcoma, tonsil
Cellular Localization of Antibody V9 Staining: Cytoplasmic
Buffer and Stabilizer: PBS with 1% BSA and 0.05% NaN3
Antibody Concentration: Lot specific. Plese contact tech support for data.
Immunogen: BALB/C mice were injected with purified vimentin from porcine eye lens.
Storage Conditions: This antibody should be stored refrigerated (2-8°C). This product should not be used past the expiration date printed on the vial.

Vimentin Information for Pathologists

Summary:

Intermediate filament for mesenchymal tissue. Uses by pathologists Vimentin staining confirms mesenchymal origin of some tumors; may be the only positive stain and thus confirms that the tissue is capable of staining (Am J Surg Pathol 2011;35:1722). Staining may confirm mesenchymal origin, but numerous exceptions so relatively nonspecific. Help distinguish renal cell clear cell carcinoma from mimics as part of panel.

Common Uses By Pathologists:

Vimentin staining confirms mesenchymal origin of some tumors; may be the only positive stain and thus confirms that the tissue is capable of staining (Am J Surg Pathol 2011;35:1722). Staining may confirm mesenchymal origin, but numerous exceptions so relatively nonspecific. Help distinguish renal cell clear cell carcinoma from mimics as part of panel. Absence of vimentin staining may confirm that fat-like or other spaces actually lack a cellular lining (Am J Surg Pathol 2011;35:1823). Distinguish endocervical and endometrial adenocarcinoma, as part of panel (Am J Surg Pathol 2010;34:915).

Limitations and Warranty

This antibody is manufactured in accordance with clinical good manufacturing practices in an ISO13485:2016 certified production facility. It is intended for multiple uses including in vitro diagnostic use and research use only applications. Please see vial label for expiration date. We strive to always deliver antibodies with a shelf life of at least two years.

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Vimentin [V9] Antibody (cGMP).