Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Monoclonal Antibody (IIIE2.34)
Formalin-fixed, paraffin-embedded human Tonsil stained with vWF Monoclonal Antibody (IIIE2.34)

Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody Clone IIIE2.34

$ 429.00
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SKU: 7450-MSM4
Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC)
Available Conjugates: Unconjugated
Isotype: Mouse IgG1 kappa
Mass Spec Validated?: Not MS Validated

Product NumberDescriptionPrice
7450-MSM4-P1 Size: 100 ug, Tag: Unconjugated
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In Stock.
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SKU: 7450-MSM4-P1
von Willebrand Factor / Factor VIII Related-Ag General Information
Alternate Names
Von Willebrand Factor, vWF
Molecular Weight
250kDa
Chromosomal Location
12p13.31
Curated Database and Bioinformatic Data
Gene SymbolVWF
Entrez Gene ID7450
Ensemble Gene IDENSG00000110799
RefSeq Protein Accession(s)NP_000543
RefSeq mRNA Accession(s)NM_000552,
RefSeq Genomic Accession(s)NC_000012, NC_018923, NG_009072
UniProt ID(s)P04275
UniGene ID(s)P04275
HGNC ID(s)12726
Cosmic ID(s)VWF
KEGG Gene ID(s)hsa:7450
PharmGKB ID(s)PA37337
General Description of von Willebrand Factor / Factor VIII Related-Ag.
von Willebrand Factor (vWF) is a multimeric glycoprotein that is found in endothelial cells, plasma, platelets. It acts as a carrier protein for Factor VIII, promotes platelet adhesion, aggregation. vWF undergoes a variety of posttranslational modifications that influence the affinity, availability for Factor VIII, including cleavage of the propeptide, formation of N-terminal disulfide bonds. This antibody helps to establish the endothelial nature of some lesions of disputed histogenesis, e.g. Kaposis sarcoma, cardiac myxoma. It is widely used for differentiating vascular lesions from those of other tissue differentiation within a panel of other vascular markers although not all tumors of endothelial differentiation contain this antigen.

Human Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody Product Attributes

Species: Human
Tested Applications: Flow Cytometry, Immunofluorescence, Western Blot, Immunoprecipitation, Immunohistochemistry (IHC).
Application Notes: Flow Cytometry (0.5-1ug of antibody/million cells in 0.1ml), Immunofluorescence (0.5-1ug of antibody/ml), Western Blot (0.5-1.0ug of antibody/ml), Immunoprecipitation (0.5-1 ug of antibody/500ug protein Lysate), Immunohistochemistry (IHC) (Formalin-fixed) (0.5-1.0ug of antibody/ml for 30 minutes at RT)
Clonality: Monoclonal
Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody Clone: IIIE2.34
Clone IIIE2.34 Host and Isotype: Mouse IgG1 kappa
Anti-Human von Willebrand Factor / Factor VIII Related-Ag Positive Control Sample: HUVEC cells or Tonsil
Cellular Localization of Antibody Cytoplasmic
Buffer and Stabilizer: 10mM PBS with 0.05% BSA & 0.05% azide.
Antibody Concentration: 200ug/ml
Antibody Purification Method:Protein A/G Purified
Immunogen: Recombinant human vWF fragment spanning aa 845-949
Storage Conditions: Store at 2 to 8° C (refrigerate). Stable for 24 months when properly stored.

von Willebrand Factor / Factor VIII Related-Ag Previously Observed Antibody Staining Patterns

Observed Antibody Staining Data By Tissue Type:

Variations in von Willebrand Factor / Factor VIII Related-Ag antibody staining intensity in immunohistochemistry on tissue sections are present across different anatomical locations. An intense signal was observed in endothelial cells in the cerebral cortex, colon. More moderate antibody staining intensity was present in endothelial cells in the cerebral cortex, colon. Low, but measureable presence of von Willebrand Factor / Factor VIII Related-Ag could be seen infibroblasts in skin, keratinocytes in skin, Langerhans in skin, melanocytes in skin, adipocytes in mesenchymal tissue and fibroblasts in mesenchymal tissue. We were unable to detect von Willebrand Factor / Factor VIII Related-Ag in other tissues. Disease states, inflammation, and other physiological changes can have a substantial impact on antibody staining patterns. These measurements were all taken in tissues deemed normal or from patients without known disease.

Limitations and Warranty

enQuire Bio's von Willebrand Factor / Factor VIII Related-Ag Anti-Human Monoclonal is available for Research Use Only. This antibody is guaranteed to work for a period of two years when properly stored.

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Anti-von Willebrand Factor / Factor VIII Related-Ag Antibody Clone IIIE2.34

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